Isolation and characterization of peroxidase from potato leaves Solanum tuberosum: Application in glucose diagnostic kit.

Hassan M M Masoud, Mohammed M Abdel-Monsef, Mohamed S Helmy, Sayed S Esa, Doaa A Darwish

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Article in Journal, genetic engineering & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Transcriptomic and Weighted Gene Co-Expression Network Analysis Reveals Molecular Regulatory Mechanisms of Cold Stress in Rice.Genes · 2026
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5 · Who and what money

Authors and funding

5 authors.

Hassan M M MasoudMolecular Biology Department, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt; Proteome Research Laboratory, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt. Electronic address: hm.massoud@nrc.sci.eg.

Mohammed M Abdel-MonsefMolecular Biology Department, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt; Proteome Research Laboratory, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt.

Mohamed S HelmyMolecular Biology Department, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt; Proteome Research Laboratory, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt.

Sayed S EsaMolecular Biology Department, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt; Proteome Research Laboratory, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt.

Doaa A DarwishMolecular Biology Department, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt; Proteome Research Laboratory, National Research Centre, El-Tahrir St., Dokki, Giza P.O. 12622, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peroxidases play a pivotal role in many medical applications such as diagnostic kits and ELISA assays. This study reports the purification and biochemical characterization of peroxidase from potato leaves (PLPOD) and its application in the formulation of a glucose diagnostic kit. PLPOD was purified through CM-cellulose ion-exchange and Sephacryl S-300 gel filtration chromatography, achieving an 11.8-fold purification with 48 % recovery and a final specific activity of 705.7 U/mg. Native PAGE and activity staining confirmed the enzyme's purity and homogeneity. PLPOD molecular weight was estimated from gel filtration column as 64 kDa, but on SDS gel, there were three PLPOD isoforms of approximated molecular weights ranging from ∼ 40-60 kDa. PLPOD exhibited optimal activity at pH 5.2, with Zn

Indexed as

CharacterizationGlucose diagnostic kitPeroxidasePotato LeavesPurification

Identifiers

PMID41386848

DOI10.1016/j.jgeb.2025.100584

PMCPMC12516557

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