ArticleBio-protocol2025
Imaging the Entire Sexual Life Cycle of the Budding Yeast
Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
Microbial life cycles are often reconstructed theoretically from fragmentary pieces of evidence. Protocols for the direct and continuous observation of entire microbial life cycles, including sexual reproduction, are scarce, which limits the study of cellular transitions between different life cycle stages and prevents the visualization of cryptic stages. Although sequence-based techniques, such as -omics approaches, can reconstruct cellular transitions at the genetic and biochemical level, these methods are destructive and do not recover information from the same living cell over time. This protocol provides a solution to directly and continuously observe microbial life cycles, including sexual reproduction, by using microfluidics manipulations that expose single cells to nutritional stimuli and selective pressures. As proof of principle, we triggered a life cycle sequence transition in the model yeast
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