ArticleFrontiers in veterinary science2025
Nanozyme conjugated chitosan nanoparticles improve buffalo bull sperm cryopreservation by enhancing antioxidant defense and mitochondrial function.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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7 authors.
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Abstract
Introduction: Cryopreservation is commonly used to preserve fertility and support genetic improvement in livestock. However, it often compromises sperm function and quality due to the excessive generation of oxidative stress. Nanozymes, a cutting-edge development in nanotechnology, offer a versatile and promising tool for mitigating oxidative stress caused by cryo-injury. This study targeted to assess the protective effects of selenium conjugated chitosan nanoparticles (SeCN; as a nanozyme) when added to semen freezing extenders in buffalo bulls. Methods: Semen samples were extended with 0 (SeCN0), 0.5 (SeCN0.5), 1 (SeCN1), or 2 (SeCN2) μg/mL SeCN, frozen at -196 °C, and assessed post-thaw for sperm quality, antioxidant status, mitochondrial activity and ultrastructural changes. Results: The SeCN supplementation significantly improved all post-thawed sperm parameters in a dose-dependent manner ( Discussion: At a concentration of 1 or 2 μg/mL, SeCN demonstrates potent cryoprotective effects by enhancing sperm function, reducing oxidative stress, and preserving mitochondrial activity and ultrastructure changes of sperm. Incorporating SeCN into semen extenders may improve cryosurvival in buffalo and represents a promising strategy for optimizing artificial insemination outcomes in livestock breeding programs.
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