Evidence map›Paper›PMID 41383705›Full record

ArticleFrontiers in molecular biosciences2025

Identification of lysozyme in Venetin-1 nanoparticle from the coelomic fluid of the earthworm

Marta J Fiołka, Magdalena Dryglewska, Kinga Lewtak, Michał Rawski, Tomasz Buchwald, Ewa Skwarek, Sachin Kote, Jakub Faktor, Weronika Ścibek-Rejmontowska, Paulina Czaplewska and 1 more

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Article in Frontiers in molecular biosciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marta J FiołkaDepartment of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
Magdalena DryglewskaDepartment of Rheumatology and Connective Tissue Diseases, Medical University of Lublin, Lublin, Poland.
Kinga LewtakDepartment of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
Michał RawskiSOLARIS National Synchrotron Radiation Centre, Jagiellonian University, Kraków, Poland.
Tomasz BuchwaldFaculty of Materials Science and Technical Physics, Institute of Materials Research and Quantum Engineering, Poznań University of Technology, Poznań, Poland.
Ewa SkwarekDepartment of Radiochemistry and Environmental Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University, Lublin, Poland.
Sachin KoteInternational Center for Cancer Vaccine Science ICCVS, University of Gdańsk, Gdańsk, Poland.
Jakub FaktorInternational Center for Cancer Vaccine Science ICCVS, University of Gdańsk, Gdańsk, Poland.
Weronika Ścibek-RejmontowskaIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
Paulina CzaplewskaIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
Wojciech KaźmierskiBruker Polska Sp. z o.o., Poznań, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: In the present study, lysozyme was analyzed in the nanoparticle Venetin-1 obtained from the coelomic fluid (CF) of the earthworm Methods: The studies were conducted using methods such as electrophoretic separation, immunodetection, and ELISA determination of lysozyme concentration. Spectroscopic methods such as FTIR and Raman spectroscopy were also employed. Proteomic analyses, SageELF separation, and zeta potential determination were also performed. Cryo-EM was used to determine the molecular structure of Venetin-1. Results: The lysozyme-type activity in this compound was significantly higher than in the crude CF. The analysis showed the highest concentration of lysozyme recognized by antibodies directed against human lysozyme is in the fraction containing compounds with a mass above 100 kDa and in the preparation obtained using a cut-off point of 6-8 kDa. Raman analysis showed a significant similarity of the protein part of Venetin-1 and EWL. FTIR analyses confirmed that the preparation obtained under these conditions showed high similarity to egg white lysozyme (EWL). Cryo-EM studies revealed the structure of the tested nanoparticle containing both lysozyme and lysenin fragments. Immunoblotting using antibodies directed against human lysozyme revealed proteins of mass 11, 33, 44, 88 and 132 kDa recognized in Venetin-1. Proteomic analyses confirmed the presence of these proteins and similarity to human lysozyme. Conclusion: These observations suggest the presence of polymeric forms of lysozyme in the tested complex, and zeta potential analysis revealed properties of the nanoparticle that predispose it to use in medicine.

Indexed as

coelomic fluidearthwormsimmunodetectionlyseninelysozymeVenetin-1

Identifiers

PMID41383705
PMCPMC12689324

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