Evidence map›Paper›PMID 41381543›Full record

ArticleScientific reports2025

A workflow integrating organ-on-chip culture and correlative 3D light and electron microscopy for microtissue analysis.

Judith M Schaart, Dorothee Wasserberg, Marcos A Eufrásio Cruz, Mariska Kea-Te Lindert, Robin H M van der Meijden, Rona Roverts, Nataliya Debera, Minh Phu Lu, Jeroen Rouwkema, Wouter H Nijhuis and 4 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Judith M SchaartDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands.
Dorothee WasserbergDepartment of Molecules and Materials, Laboratory of Biointerface Chemistry, Organ-on-Chip Centre, TechMed Centre and MESA+ Institute, University of Twente, Drienerlolaan 5, 7522NB, Enschede, The Netherlands.
Marcos A Eufrásio CruzDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands.
Mariska Kea-Te LindertDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands.
Robin H M van der MeijdenDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands.
Rona RovertsDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands.
Nataliya DeberaDepartment of Molecules and Materials, Laboratory of Biointerface Chemistry, Organ-on-Chip Centre, TechMed Centre and MESA+ Institute, University of Twente, Drienerlolaan 5, 7522NB, Enschede, The Netherlands.
Minh Phu LuDepartment of Molecules and Materials, Laboratory of Biointerface Chemistry, Organ-on-Chip Centre, TechMed Centre and MESA+ Institute, University of Twente, Drienerlolaan 5, 7522NB, Enschede, The Netherlands.
Jeroen RouwkemaDepartment of Biomechanical Engineering, University of Twente, Drienerlolaan 5, 7522NB, Enschede, The Netherlands.
Wouter H NijhuisDepartment of Orthopaedic Surgery, University Medical Centre Utrecht, Wilhelmina Children's Hospital, Lundlaan 6, 3548EA, Utrecht, The Netherlands.
Andries D van der MeerDepartment of Bioengineering Technologies, University of Twente, Drienerlolaan 5, 7522NB, Enschede, The Netherlands.
Pascal JonkheijmDepartment of Molecules and Materials, Laboratory of Biointerface Chemistry, Organ-on-Chip Centre, TechMed Centre and MESA+ Institute, University of Twente, Drienerlolaan 5, 7522NB, Enschede, The Netherlands. p.jonkheijm@utwente.nl.
Nico SommerdijkDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands. nico.sommerdijk@radboudumc.nl.
Anat AkivaDepartment of Medical BioSciences, Radboud University Medical Center, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands. anat.akiva@radboudumc.nl.

Funding

HORIZON EUROPE European Research Council H2020-ERC-2017-ADV-788982-COLMINNederlandse Organisatie voor Wetenschappelijk Onderzoek OCENW.GROOT.2019.029Nederlandse Organisatie voor Wetenschappelijk Onderzoek VI.Veni.192.094Topconsortium voor Kennis en Innovatie CHEMIE.PGT.2023.017
6 · The paper itself

Abstract

Correlative microscopy approaches offer powerful means to study tissue development across spatial scales, but combining 3D light and electron imaging remains technically challenging. Here, we present a practical workflow that integrates organ-on-a-chip culture with longitudinal fluorescence imaging and volume electron microscopy. By modifying an existing chip platform designed for aligned tissue growth, we demonstrate the feasibility of extended 3D live imaging and subsequent high-pressure freezing of intact microtissues. Fluorescence-guided targeting enables focused ion beam/scanning electron microscopy (FIB/SEM) of selected regions, revealing ultrastructural features such as cellular organization, collagen alignment, and matrix mineralization. While not aimed at new biological discoveries, this study highlights the compatibility and potential of this pipeline for future high-resolution, multiscale studies of tissue morphogenesis and pathology in controlled microenvironments.

Indexed as

Imaging, Three-DimensionalLab-On-A-Chip DevicesAnimalsHumansMicrophysiological SystemsMicroscopy, Electron, ScanningMicroscopy, FluorescenceVolume Electron MicroscopyWorkflowBone-on-a-chipCorrelative light and electron microscopyLive fluorescence microscopyOrgan-on-a-chipVolume electron microscopy

Identifiers

PMID41381543
PMCPMC12700958

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.