Evidence map›Paper›PMID 41381532›Full record

ArticleNature communications2025

Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes.

Doron S Y Zaada, Or Toren, Flavia Krsticevic, Daniella A Haber, Denys Gildman, Noam Galpaz, Irina Häcker, Marc F Schetelig, Eric Marois, Yael Arien and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Doron S Y ZaadaDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.ORCID http://orcid.org/0009-0004-6616-0098
Or TorenDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
Flavia KrsticevicDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
Daniella A HaberDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.ORCID http://orcid.org/0009-0000-1349-3412
Denys GildmanDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
Noam GalpazDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.ORCID http://orcid.org/0009-0001-9194-7667
Irina HäckerDepartment of Insect Biotechnology in Plant Protection, Institute for Insect Biotechnology, Justus Liebig University of Giessen, Winchesterstrasse. 2, 35394, Giessen, Germany.ORCID http://orcid.org/0000-0003-4022-4853
Marc F ScheteligDepartment of Insect Biotechnology in Plant Protection, Institute for Insect Biotechnology, Justus Liebig University of Giessen, Winchesterstrasse. 2, 35394, Giessen, Germany.ORCID http://orcid.org/0000-0002-9217-394X
Eric MaroisInstitut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, INSERM, CNRS, Strasbourg, France.ORCID http://orcid.org/0000-0003-4147-3747
Yael ArienDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
Philippos A PapathanosDepartment of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel. p.papathanos@mail.huji.ac.il.ORCID http://orcid.org/0000-0001-8508-4554

Funding

Agence Nationale de la Recherche (French National Research Agency) ANR-23-CE35-0003Deutsche Forschungsgemeinschaft (German Research Foundation) 1833/7-1EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 101059523Ministry of Science, Technology and Space 3-1679
6 · The paper itself

Abstract

Effective sex separation remains a critical challenge for mosquito genetic control. Genetic sexing strains (GSS) address this by linking maleness with selectable traits, enabling efficient female removal. Here, we present a versatile platform for GSS development in the invasive Aedes albopictus mosquito that integrates CRISPR-engineered selectable phenotypes with sex conversion via nix, the male-determining factor. As a proof-of-concept, we disrupt the yellow pigmentation gene and restore its function in males using nix-containing transgenes, producing a stable strain with yellow females and dark males. Beyond serving as a vivid marker, yellow confers added advantages: GSS females pupate later than wild females, enhancing protandry-based sorting, and lay desiccation-sensitive eggs, lowering accidental female release risk. The strain is compatible with size-based separation, improving sexing accuracy through the integration of natural and engineered dimorphisms. To our knowledge, this represents the first engineered sex-linked selectable trait in mosquitoes based on endogenous genes, establishing a foundation for scalable GSS development.

Indexed as

AedesSex ChromosomesSex Determination ProcessesAnimalsAnimals, Genetically ModifiedCRISPR-Cas SystemsFemaleGenetic EngineeringInsect ProteinsMalePhenotypePigmentationInsect Proteins

Identifiers

PMID41381532
PMCPMC12711996

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.