Evidence map›Paper›PMID 41380835›Full record

ArticleJournal of advanced research2026

Extracellular matrix-inspired multi-morphology microspheres harness osteogenic-prone stem cells to recruit and reprogram adjacent stem cells for synergistic bone regeneration.

Huixin Lv, Yihan Wang, Jingxia Chen, Yangfan Pei, Xiuyu Liu, Siyu Chen, Sheng Chen, Yanmin Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Bone organoids and mitochondrial reprogramming.Journal of orthopaedic translation · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Huixin LvDepartment of Prosthodontics, Peking University School and Hospital of Stomatology, 100081 Beijing, China; National Center for Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices and Beijing Key Laboratory of Digital Stomatology and National Health Commission Key Laboratory of Digital Technology of Stomatology, 100081 Beijing, China; Institute of Advanced Clinical Medicine, Peking University, 100191 Beijing, China. Electronic address: lvhx@bjmu.edu.cn.
Yihan WangDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: yhw22@mails.jlu.edu.cn.
Jingxia ChenDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: jingxia23@mails.jlu.edu.cn.
Yangfan PeiDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: peiyf24@mails.jlu.edu.cn.
Xiuyu LiuDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: xiuyu24@mails.jlu.edu.cn.
Siyu ChenDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: siyuc22@mails.jlu.edu.cn.
Sheng ChenDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: chensheng22@mails.jlu.edu.cn.
Yanmin ZhouDepartment of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, China. Electronic address: zhouym@jlu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe limited proportion and age-related decline in proliferative capacity of skeletal stem cells within jawbone tissue hinder efficient bone regeneration. Enhancing osteogenesis through stem cell recruitment and phenotypic modulation via intercellular communication remains an underexplored strategy. We hypothesized that osteogenic-prone stem cells can recruit adjacent osteogenic-weak cells and promote their proliferation and osteogenic differentiation. Biomimetic scaffolds mimicking an extracellular matrix (ECM) microenvironment favorable to osteogenic-prone stem cells may amplify this intercellular cascade.

methodsBased on our previous findings of two osteogenically heterogeneous stem cell populations with intercellular communication potential, we designed biomimetic mineralized (methacrylated gelatin)-fibrinogen crosslinked hydrogel microspheres (bmGFMs) via microemulsion and photocrosslinking, guided by the gene signatures of the osteogenic-prone stem cell subtype. These microspheres supported multidirectional cell growth and were used to construct a 3D culture system in which distinct stem cell populations were loaded separately under contact or non-contact conditions. The proliferative, migratory, and osteogenic capacities of the cells were examined using RNA-seq, qRT-PCR, western blot, immunofluorescence, subcutaneous ectopic transplantation, etc. In vivo study was conducted in rabbits undergoing bilateral bone augmentation in the maxillary posterior region, and regeneration outcomes were assessed via micro-CT, H&E staining, immunofluorescence, etc.

resultsThe bmGFMs provided physicochemical and mechanical cues that preferentially recruited osteogenic-prone stem cells for colonization and osteogenesis via FAK/PKC/PI3K/Akt signaling pathway. Enhanced interstitial fluid infiltration facilitated paracrine signaling (e.g., SDF-1) from these primed cells, recruiting nearby osteogenic-weak populations and inducing their osteogenic conversion, thereby expanding osteogenic source. In vivo, bmGFMs guided spatiotemporal bone formation, achieving high-density intramembranous ossification through dual-cell synergy.

conclusionHarnessing endogenous stem cell cooperation to enrich osteogenic sources offers a promising strategy for jawbone regeneration. Developing biomimetic materials tailored to genetic profiles of resident osteogenic-prone stem cells to sequentially enhance stemness maintenance and osteogenic differentiation provides a potent collaborative approach for bone tissue engineering.

Indexed as

Bone RegenerationExtracellular MatrixMicrospheresOsteogenesisStem CellsAnimalsBiomimetic MaterialsCell DifferentiationCell ProliferationHumansHydrogelsMesenchymal Stem CellsRabbitsTissue EngineeringTissue ScaffoldsHydrogelsAdult stem cellsBiomimetic materialsBiomimetic mineralizationBone regenerationCell communication

Identifiers

PMID41380835
PMCPMC13539149

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.