Evidence map›Paper›PMID 41380756›Full record

ArticleInternational journal for parasitology2026

Genetic signatures of  Plasmodium vivax Circumsporozoite Surface Protein during malaria resurgence in Thailand.

Parsakorn Tapaopong, Aurel Holzschuh, Gustavo da Silva, Palakorn Chintanawiwat, Sirasate Bantuchai, Wasinee Rungsarityotin, Liwang Cui, Cristian Koepfli, Jetsumon Sattabongkot, Wang Nguitragool

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In one paragraph

Article in International journal for parasitology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Parsakorn TapaopongMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Aurel HolzschuhDepartment of Medicine, Swiss Tropical and Public Health Institute, Allschwil, Switzerland; University of Basel, Basel, Switzerland.
Gustavo da SilvaDepartment of Biological Sciences, Eck Institute for Global Health, Galvin Life Sciences, University of Notre Dame, Notre Dame, IN, USA.
Palakorn ChintanawiwatMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Sirasate BantuchaiMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Wasinee RungsarityotinMahidol University Frontier Research Facility, Mahidol University, Salaya, Nakhon Pathom, Thailand.
Liwang CuiDivision of Infectious Diseases and International Medicine, Department of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Cristian KoepfliDepartment of Biological Sciences, Eck Institute for Global Health, Galvin Life Sciences, University of Notre Dame, Notre Dame, IN, USA.
Jetsumon SattabongkotMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Wang NguitragoolMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. Electronic address: wang.ngu@mahidol.ac.th.

Funding

The changing landscape of human and zoonotic malaria in Southeast AsiaU19AI181583 · NIAID · MAHIDOL UNIVERSITY · PI Amnat Khamsiriwatchara · 2024 to 2026
$3.6M
NIAID NIH HHS U19 AI181583
6 · The paper itself

Abstract

The Plasmodium circumsporozoite surface protein (CSP) is the best characterized pre-erythrocytic vaccine target for malaria. It is a multifunctional protein important for sporozoite mobility, mosquito salivary gland invasion, and hepatocyte invasion. We analyzed diversity of  Plasmodium vivax CSP gene (pvcsp) during the 2022-2023 malaria resurgence in northwestern Thailand and assessed how pvcsp haplotypes may affect parasite development in the mosquitoes. Amplicon sequencing of 69 P. vivax isolates revealed both canonical pvcsp variants: VK210 (n = 66) and VK247 (n = 3). The VK210 type exhibited high polymorphism within the central repeat region, with 21 haplotypes (H1-H21) composed of 13-20 repeat motifs. Haplotype H2 was the most common, accounting for half of all VK210 sequences, and in membrane feeding assays with Anopheles dirus, appeared to produce more salivary-gland sporozoites per oocyst than other haplotypes, suggesting that repeat-region variation may modulate vector competence. Together, these findings report contemporary pvcsp diversity in Thailand's highest transmission area, provide functional evidence that repeat-region polymorphisms shape vector-parasite interactions, and highlight three globally prevalent motifs (GDRADGQPA, GDRAAGQPA, ANGAGNQPG) as prime targets for future PvCSP vaccines.

Indexed as

Malaria, VivaxPlasmodium vivaxProtozoan ProteinsAnimalsAnophelesGenetic VariationHaplotypesHumansPolymorphism, GeneticSporozoitesThailandcircumsporozoite protein, ProtozoanProtozoan ProteinsCircumsporozoite surface proteinGenetic diversityMalariaPlasmodium vivaxThailand

Identifiers

PMID41380756
PMCPMC12949852

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.