Evidence map›Paper›PMID 41379305›Full record

ArticleJournal of endocrinological investigation2026

Characterizing hypothalamic damage patterns in pigs with metabolic disorders by transcriptome analysis.

Huanqi Peng, Kaiyi Zhang, Jiakun Miao, Yu Yang, Shuang Xu, Tianwen Wu, Cong Tao, Yanfang Wang, Shulin Yang

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of endocrinological investigation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Huanqi PengState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.ORCID http://orcid.org/0000-0002-9725-8117
Kaiyi ZhangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Jiakun MiaoState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Yu YangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Shuang XuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Tianwen WuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Cong TaoState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Yanfang WangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Shulin YangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. yangshulin@caas.cn.

Funding

Agricultural Science and Technology Innovation Program ASTIP-IAS-05Key Technologies Research and Development Program 2021YFA0805903National Natural Science Foundation of China 32070535National Science and Technology Major Project 2023ZD04072National Science Fund for Distinguished Young Scholars 32025034
6 · The paper itself

Abstract

purposeThe hypothalamus serves as a crucial regulator of energy homeostasis, and accumulating research shows that defects in the hypothalamus are implicated in the link between type 2 diabetes mellitus (T2DM) and obesity. This study was designed to explore the patterns of hypothalamic damage under conditions of metabolic disorders through transcriptome sequencing.

methodsFive wild-type pigs (WT) and five transgenic pigs (PIGinH11) of comparable ages and body weights were selected for this study. Both groups were fed a high-fat, high-sucrose diet (HFHSD) for 12 weeks. Subsequently, serological parameters were evaluated, and RNA sequencing was carried out on hypothalamic tissues. The transcriptome sequencing results were verified by RT‒PCR and immunofluorescence experiments.

resultsA total of 561 differentially expressed genes were identified in the PIGinH11 pigs, with 176 genes being upregulated and 381 genes being downregulated. Protein‒protein interaction (PPI) analysis revealed that the top differentially expressed genes could be grouped into three clusters, namely, vasoconstriction and dilation, inflammation, and metabolism. The genes CD8A, HDG, and NOS1 might be the core genes of each cluster. The KEGG pathway enriched by Gene Set Enrichment Analysis (GSEA) results revealed that pathways related to protein synthesis-related inflammation were upregulated in PIGinH11 pigs, whereas those associated with calcium homeostasis were downregulated.

conclusionThe results of the present study demonstrated that in PIGinH11 pigs, which are sensitive to energy alterations, the hypothalamus exhibited inflammation, calcium dysregulation, and activated protein synthesis at the transcriptomic level under metabolic disorders. The NO-sGC-cGMP pathway may play a critical role in hypothalamic inflammation, warranting further in-depth investigation.

Indexed as

HypothalamusMetabolic DiseasesTranscriptomeAnimalsAnimals, Genetically ModifiedDiet, High-FatGene Expression ProfilingSwineHypothalamusObesityPig model(s)RNA-seq

Identifiers

PMID41379305

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.