Evidence map›Paper›PMID 41379153›Full record

ArticleDie Naturwissenschaften2025

A comparative analysis of bioactive phytochemicals in Cynanchum viminale (L.) L. and Pergularia daemia (Forssk.) Chiov. from Saudi Arabian folk medicine.

Nawaf I Alsenani, Saif H Althagafi, Abdelazim Ali Ahmed, Ahmed A Elhenawy, Ohoud J AAlotaibi, Fatemah M Asiri, Nujud H Alqahtani, Sameera N Al-Ghamdi, Nada M Ali, Amal A Alageel and 2 more

Abstract readComparative Study
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In one paragraph

Article in Die Naturwissenschaften, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Nawaf I AlsenaniChemistry Department, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia.
Saif H AlthagafiChemistry Department, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia. salthaqafi@bu.edu.sa.
Abdelazim Ali AhmedMinistry of Environment, Water and Agriculture, National Center for Vegetation Cover Development and Combating Desertification (NCVC), Riyadh, Saudi Arabia.
Ahmed A ElhenawyChemistry Department, Faculty of Science, Al-Azhar University, Nasr City, 11884, Cairo, Egypt.
Ohoud J AAlotaibiDepartment of Chemistry Sciences, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
Fatemah M AsiriChemistry Department, College of Science & Arts, Najran University, Najran, Saudi Arabia.
Nujud H AlqahtaniDepartment of Chemistry, College of Science, King Khalid University, Abha, Saudi Arabia.
Sameera N Al-GhamdiChemistry Department, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia.
Nada M AliChemistry Department, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia.
Amal A AlageelChemistry Department, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia.
Antar A AbdelhamidChemistry Department, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia.
Fawy M Abd El LatifChemistry Department, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The dual global health crises of antimicrobial resistance and cancer demand the urgent discovery of novel therapeutic leads. The medicinal plants Cynanchum viminale and Pergularia daemia (Apocynaceae) represent a rich reservoir of bioactive compounds used in traditional medicine, yet a comprehensive comparative analysis of their bioactivities and molecular mechanisms has remained elusive. This study provides an integrated in vitro and in silico analysis of the methanolic extracts of C. viminale and P. daemia. Phytochemical profiles were delineated by HPLC. Bioactivities were systematically evaluated via a battery of five antioxidant assays (DPPH, ABTS, FRAP, H₂O₂, NO), broad-spectrum antimicrobial screening against six pathogens, and cytotoxicity assays against a panel of six human cancer cell lines (HepG-2, MCF-7, HCT-116, A-549, PC-3, A-431). To deconstruct the molecular basis of these activities, in silico molecular docking was performed against two pivotal therapeutic targets: Epidermal Growth Factor Receptor (EGFR) and Dihydrofolate Reductase (DHFR). A striking functional divergence between the two plants was discovered. P. daemia exhibited superior antioxidant and broader-spectrum antibacterial activity, a finding strongly correlated with its unique phenolic profile, particularly the potent DHFR-binding Chlorogenic acid and the bioenhancing alkaloid Piperine. In stark contrast, C. viminale demonstrated dramatically superior cytotoxic potency across all tested cancer cell lines, with IC₅₀ values as low as 24.37 µg/mL against HepG-2 liver cancer. Molecular docking brilliantly illuminated the mechanism behind this divergence: the potent cytotoxicity of C. viminale is driven by its principal alkaloids, Protopine and Berberine, which showed high-affinity binding to the ATP-binding site of EGFR, a key driver of cancer proliferation. DHFR, conversely, was identified as a common molecular target for potent binders from both plants (Evodiamine, Piperine, Chlorogenic acid), providing a unifying mechanism for their shared antimicrobial properties.

Indexed as

Medicine, TraditionalPhytochemicalsPlant ExtractsAnti-Infective AgentsAntioxidantsCell Line, TumorHumansMolecular Docking SimulationSaudi ArabiaAnti-Infective AgentsAntioxidantsPhytochemicalsPlant ExtractsBioactivityC. viminaleMedicinal plantsP. daemiaPhytoconstituents

Identifiers

PMID41379153

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.