Evidence map›Paper›PMID 41378780›Full record

ArticleMolecular pain

Mast cell-5-HT-HTR2A axis involvement in chronic itch induced by SADBE.

Limin Fan, Xiuyu Nong, Manting Ni, Xi Chen, Liping Zeng, Huifang Chen, Jian Wang, Xuan Ouyang, Ailin Tao, Xueting Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular pain. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Limin FanThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.ORCID 0009-0000-6836-1257
Xiuyu NongThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Manting NiThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Xi ChenThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Liping ZengThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Huifang ChenThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Jian WangState Key Laboratory of Respiratory Diseases, National Center for Respiratory Medicine, Guangdong Key Laboratory of Vascular Diseases, National Clinical Research Center for Respiratory Diseases, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Xuan OuyangState Key Laboratory of Respiratory Diseases, National Center for Respiratory Medicine, Guangdong Key Laboratory of Vascular Diseases, National Clinical Research Center for Respiratory Diseases, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Ailin TaoThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Xueting LiuThe Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, GMU-GIBH Joint School of Life Sciences of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.ORCID 0000-0001-5419-198X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although 5-hydroxytryptamine (5-HT) contributes to pruritus associated with allergic contact dermatitis (ACD), the role of 5-HT derived from mast cells (MC) in chronic pruritus induced by squaric acid dibutyl ester (SADBE), and the expression and distribution of 5-HT2A receptor (HTR2A) in sensory neurons remain unclear. In this study, a SADBE-induced ACD mouse model was established to evaluate pruritus behavior, MC activation, and 5-HTproduction. The mechanism was verified through pharmacological intervention (MC stabilizer cromolyn, HTR2A antagonist Ketanserin) and FcεRIα-KO mice. It was found that SADBE triggered time-dependent MC recruitment (peaking at Day 14-21) and Mc-derived 5-HT release, which were associated with persistent pruritus. The intervention of MC stabilizer cromolyn and FcεRIα-KO mice confirmed MC/IgE-dependent 5-HT release, and inhibiting MC degranulation could reduce pruritus. Single-cell RNA sequencing and RNAscope in situ hybridization techniques revealed that HTR2A was mainly expressed in the NF3/PEP2/NP3 subsets of DRG neurons. The co-expression level of HTR2A and Nppb was relatively high, partially overlapping with TRPV1/TRPA1. HTR2A antagonists can relieve SADBE-induced pruritus. In conclusion, we have determined that the MC-5-HT-HTR2A axis is involved in chronic pruritus in SADBE-induced ACD, and targeting this axis provides a very promising therapeutic strategy.

Indexed as

CyclobutanesMast CellsPruritusReceptor, Serotonin, 5-HT2ASerotoninAnimalsCell DegranulationChronic DiseaseDisease Models, AnimalMaleMiceMice, Inbred C57BLMice, KnockoutReceptors, IgESensory Receptor CellsCyclobutanesReceptor, Serotonin, 5-HT2AReceptors, IgESerotonin5-HTallergic contact dermatitisdorsal root ganglionHTR2AItchmast cells

Identifiers

PMID41378780
PMCPMC12833167

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.