ReviewBiophysical reviews2025
Stabilising proteins in solution using affordable and readily available small molecules.
Review in Biophysical reviews, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Anti-fibrillation and trans-fibrillation activities of L-arginine modified magnetic nanoparticles on lysozyme.Biochemistry and biophysics reports · 2026Article
- Biophysics in Great Britain and Ireland.Biophysical reviews · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purified proteins are sitting in a mostly aqueous environment, with normally some buffer and salt making up the conditions. This is vastly different from their natural habitat, and protein are often affected by this difference, showing signs of destabilisation or aggregation. A common method to improve the protein solubility and homogeneity is adding small molecules to the buffer conditions, as these can aid protein stability and keep the protein in solution at a concentration which is within that needed for the experiments that are to be undertaken. This review is detailing some of the small molecules that are routinely used, with a focus on them being readily available and affordable for all labs. Some of the more common small molecule additives described in this paper are (1) amino acids, like arginine or glycine, (2) sugars, like sucrose, or (3) other osmolytes, such as glycerol. The second part is covering some of the methods that can be utilised to determine whether a small molecule improves the stability of a particular protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.