ReviewBiophysical reviews2025
High-throughput investigation of macromolecular interactions for drug development using spectral shift technology.
Review in Biophysical reviews, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Efficient Method for High-Throughput Screening of Compound Libraries Targeting Human PD-L1.ACS omega · 2026Article
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3 authors.
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Abstract
This review focuses on spectral shift analysis as a tool to study macromolecular interactions and describes its current place among the available biophysical methods. NanoTemper's Dianthus platform facilitates a plate-based, microfluidics-free, mass-independent, and immobilisation-free high-throughput screening platform for protein-ligand, protein-protein, and protein-nucleic acid interactions, as well as ternary complexes, for example in proteolysis targeting chimera (PROTAC) design. In addition to spectral shift, the Dianthus offers an orthogonal method, temperature-related intensity change (TRIC). Both methods are presented alongside fluorescent labelling techniques. Specific examples with practical tips for spectral shift methods for diverse binding partners are provided. Finally, current and future applications of spectral shift methods in the drug discovery process are discussed in the context of high-throughput screening, fragment-based drug discovery, and hit-to-lead optimisation.
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