Evidence map›Paper›PMID 41377508›Full record

ArticlebioRxiv : the preprint server for biology2025

Characterization of a high-intensity band that cross-reacts with FLAG-M2 antibodies in immunoblots in a subset of laboratory strains of Saccharomyces cerevisiae.

Nicolle K Reid, Amy C Liang, Mara C Duncan

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nicolle K ReidDepartment of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI.ORCID 0009-0001-9082-8690
Amy C LiangDepartment of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI.ORCID 0009-0005-6877-6889
Mara C DuncanDepartment of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI.ORCID 0000-0002-2383-0109

Funding

Michigan Institute for Clinical and Health Research (MICHR)UL1TR002240 · NCATS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LUMENG, JULIE C, MASHOUR, GEORGE ALEXANDER · 2017 to 2022
$54.9M
Text mining in the CloudU24HG010859 · NHGRI · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAROL J BULT, PAUL Warren STERNBERG · 2019 to 2026
$42.0M
Resource ProjectU41HG002273 · NHGRI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI THOMAS, PAUL D. · 2012 to 2021
$34.7M
Genomic Resource for the Yeast SaccharomycesU24HG001315 · NHGRI · STANFORD UNIVERSITY · PI Stacia Engel, Gavin J Sherlock · 2021 to 2026
$10.9M
Characterizing new proteins that determine AP-1 recruitment and distributionR01GM129255 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DUNCAN, MARA C · 2019 to 2022
$1.2M
NCATS NIH HHS UL1 TR002240NHGRI NIH HHS U24 HG001315NHGRI NIH HHS U24 HG010859NHGRI NIH HHS U41 HG002273NIGMS NIH HHS R01 GM129255
6 · The paper itself

Abstract

Epitope tags are commonly used for various purposes in research labs. The DYKDDDDK-peptide epitope, trademarked as the FLAG epitope, is a commonly used epitope tag. It is often used for monitoring protein levels and for affinity chromatography. Multiple DYKDDDDK-binding antibodies are available; however, the mouse monoclonal anti-FLAG M2 is widely used due to its commercial availability in several formats. Many laboratory

Identifiers

PMID41377508
PMCPMC12687974

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.