Evidence map›Paper›PMID 41377505›Full record

ArticlebioRxiv : the preprint server for biology2025

Construction of a randomly barcoded insertional mutant library in the filamentous fungus

Lori B Huberman, José M Villalobos-Escobedo, Jeffrey M Skerker, Ran Shi, Adriana M Rico-Ramírez, Catharine Adams, Adam P Arkin, Adam M Deutschbauer, N Louise Glass

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lori B HubermanPlant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA.ORCID 0000-0003-2638-8528
José M Villalobos-EscobedoDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0002-8412-6748
Jeffrey M SkerkerDepartment of Bioengineering, University of California, Berkeley, California, USA.ORCID 0000-0003-2653-1566
Ran ShiDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0009-0004-2436-2900
Adriana M Rico-RamírezDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0002-4196-8427
Catharine AdamsDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0002-0914-0806
Adam P ArkinEnvironmental Genomics and System Biology Division/Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.ORCID 0000-0002-4999-2931
Adam M DeutschbauerDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0003-2728-7622
N Louise GlassDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0002-4844-2890

Funding

Genetic mechanisms of signal integration in the nutrient sensing networkR35GM150926 · NIGMS · CORNELL UNIVERSITY · PI Lori B Huberman · 2023 to 2026
$1.5M
NIGMS NIH HHS R35 GM150926
6 · The paper itself

Abstract

Filamentous fungi play key roles in ecosystems, agriculture, biotechnology, symbiosis, and disease, yet the large-scale characterization of gene function in these organisms remains limited by low transformation efficiencies and their multinucleate, syncytial cells, which complicate high-throughput screening strategies. To address the challenge of high-throughput screening in filamentous fungi, we developed methods to construct a genome-wide barcoded insertional mutant library in

Indexed as

Random Barcode TDNAseqTDNA insertional mutantsTrichoderma

Identifiers

PMID41377505
PMCPMC12687792

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.