Evidence map›Paper›PMID 41374993›Full record

ArticleCancers2025

MicroRNA Profiling Identifies Diagnostic and Prognostic Markers in Pediatric Sarcoma.

Terrie G Flatt, Leonid M Yermakov, Shreeram Akilesh, Eleanor Y Chen, Elizabeth Gonzalez, Alejandro Parrales, Marta Zapata-Tarres, Rocio Cardenas-Cardos, Liliana Velasco-Hidalgo, Celso Corcuera-Delgado and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Terrie G FlattDepartment of Pediatrics, Division of Hematology & Oncology, Children's Mercy Hospital, University of Missouri, Kansas City, MO 64110, USA.
Leonid M YermakovDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-7770-1716
Shreeram AkileshDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.
Eleanor Y ChenDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0003-4372-7560
Elizabeth GonzalezDepartment of Pediatrics, Division of Hematology & Oncology, Children's Mercy Hospital, University of Missouri, Kansas City, MO 64110, USA.
Alejandro ParralesDepartment of Pediatrics, Division of Hematology & Oncology, Children's Mercy Hospital, University of Missouri, Kansas City, MO 64110, USA.
Marta Zapata-TarresResearch Coordination Mexican Institute of Social Security Foundation, Mexico City 06600, Mexico.
Rocio Cardenas-CardosDepartamento de Oncología Pediátrica, Instituto Nacional de Pediatría, Mexico City 04530, Mexico.
Liliana Velasco-HidalgoDepartamento de Oncología Pediátrica, Instituto Nacional de Pediatría, Mexico City 04530, Mexico.
Celso Corcuera-DelgadoDepartamento de Patología Pediátrica, Instituto Nacional de Pediatría, Mexico City 04530, Mexico.
Rodolfo Rodriguez-JuradoDepartamento de Patología Pediátrica, Instituto Nacional de Pediatría, Mexico City 04530, Mexico.ORCID 0000-0001-9527-4861
Lillia García-RodríguezFacultad de Medicina, Universidad de Monterrey, San Pedro Garza García, Monterrey 66238, Mexico.
Midhat S FarooqiDepartment of Pathology & Laboratory Medicine, Children's Mercy Hospital, University of Missouri, Kansas City, MO 64110, USA.ORCID 0000-0002-5238-1349
Atif Ali AhmedDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-8791-5785

Funding

Kansas Masonic Foundation N/A
6 · The paper itself

Abstract

backgroundMicroRNAs (miRNAs) function as post-transcriptional gene expression regulators and influence the development and progression of several cancers, yet their roles in pediatric sarcomas remain poorly defined.

methodsRNA extracted from formalin-fixed paraffin-embedded tumor tissue scrolls of 108 pediatric tumors, including 32 osteosarcoma (OS), 26 Ewing's sarcoma (EWS), and 50 rhabdomyosarcoma (RMS) cases, were analyzed for microRNA expression using the NanoString multiplex nCounter platform that yielded information on 827 human miRNAs. The expression of candidate miRNAs was validated with in situ hybridization (miRNA-ISH) and QuPath quantification on tissue microarray slides comprising an independent set of 48 OS, 17 EWS, and 104 RMS adult and pediatric cases collectively.

resultsThe differential expression analysis of nCounter data identified 23 miRNAs enriched in RMS, 33 in EWS, and 45 in OS (>3 fold change and

conclusionsCollectively, these findings demonstrate that distinct miRNA profiles can differentiate pediatric sarcoma types and subtypes and offer clinically relevant insights into tumor biology, prognosis, and potential diagnostic application.

Indexed as

Ewing’s sarcomamicroRNAmicroRNA in situ hybridizationNanoString nCounterosteosarcomarhabdomyosarcoma

Identifiers

PMID41374993
PMCPMC12691150

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.