Evidence map›Paper›PMID 41373768›Full record

ArticleInternational journal of molecular sciences2025

Benchmarking Cost-Effective DNA Extraction Kits for Diverse Metagenomic Samples.

Andrey Sobolev, Daria Sibiryakina, Elizaveta Chevokina, Darya Slonova, Daria Yurikova, Svetlana Kozlova, Anna Trofimova, Vasiliy Zubarev, Alexander Kiselev, Olga Konovalova and 2 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Andrey SobolevThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.ORCID 0000-0003-2941-5325
Daria SibiryakinaThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.
Elizaveta ChevokinaThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.
Darya SlonovaThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.ORCID 0000-0002-2465-0193
Daria YurikovaMarine Research Center of Lomonosov Moscow State University, Moscow 119607, Russia.
Svetlana KozlovaThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.ORCID 0000-0003-2251-0354
Anna TrofimovaThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.
Vasiliy ZubarevThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.ORCID 0009-0007-0204-6191
Alexander KiselevShirshov Institute of Oceanology, Russian Academy of Sciences, Moscow 117997, Russia.ORCID 0009-0005-5737-1480
Olga KonovalovaMarine Research Center of Lomonosov Moscow State University, Moscow 119607, Russia.ORCID 0000-0002-9023-7938
Dmitry SutorminThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.
Artem IsaevThe Center for Bio- and Medical Technologies, Moscow 121205, Russia.ORCID 0000-0002-9593-2745

Funding

Ministry of Science and Higher Education 075-10-2021-114Russian Science Foundation 22-14-00004
6 · The paper itself

Abstract

Extraction of high-quality microbial DNA remains a critical bottleneck in metagenomic research. Environmental samples often produce fragmented DNA and are prone to contaminations that interfere with downstream sequencing, while widely used commercial kits can be prohibitively expensive. Therefore, systematic evaluation of cost-effective alternatives is essential to support large-scale metagenomic studies. In this work, we benchmarked eight commercial DNA extraction kits from Magen, SkyGen, and Sileks against Qiagen reference kits. Four representative sample types were analyzed: freshwater, seafloor sediments, Pacific oyster (

Indexed as

DNADNA, BacterialMetagenomeMetagenomicsAnimalsBenchmarkingCost-Benefit AnalysisFecesGastrointestinal MicrobiomeGeologic SedimentsReproducibility of ResultsRNA, Ribosomal, 16SDNADNA, BacterialRNA, Ribosomal, 16S16S sequencingbenchmarkcost-effectivenessDNA purificationenvironmental samplesmetagenomics

Identifiers

PMID41373768
PMCPMC12692181

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.