Evidence map›Paper›PMID 41373718›Full record

ArticleInternational journal of molecular sciences2025

Multiplex Immunofluorescence Assay with Opal Reagents for Identifying Mononuclear Cell Subsets in Kidney Allograft Rejection Types.

Ernest Kwame Adjepong-Tandoh, Jin-Myung Kim, Hye Eun Kwon, Youngmin Ko, Joo Hee Jung, Young Hoon Kim, Heounjeong Go, Haeyon Cho, Sang-Yeob Kim, Yeon-Mi Ryu and 2 more

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Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ernest Kwame Adjepong-TandohDepartment of Surgery, Korle Bu Teaching Hospital, Accra P.O. Box KB-77, Ghana.
Jin-Myung KimDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Hye Eun KwonDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Youngmin KoDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Joo Hee JungDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Young Hoon KimDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Heounjeong GoDepartment of Pathology, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.ORCID 0000-0003-0412-8709
Haeyon ChoDepartment of Pathology, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Sang-Yeob KimAsan Institute for Life Sciences, Asan Medical Center, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Yeon-Mi RyuAsan Institute for Life Sciences, Asan Medical Center, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Sung ShinDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
Hyunwook KwonDivision of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.ORCID 0000-0001-5018-5304

Funding

Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea 2021IL0027-2
6 · The paper itself

Abstract

Antibody-mediated rejection (ABMR) remains a leading cause of kidney allograft failure, yet the mechanistic roles of innate immune cell subsets such as monocytes and natural killer (NK) cells remain incompletely understood. In this retrospective cohort study, we applied OPAL-based multiplex immunofluorescence (mIF) to human kidney allograft biopsies from 38 recipients with biopsy-proven ABMR (n = 19), T-cell-mediated rejection (TCMR, n = 12), or no rejection (NR, n = 7), enabling spatially resolved quantification of immune subsets in situ. Fluorescence thresholds were pathologist-validated, and co-expression phenotypes were defined using standardized segmentation and spectral unmixing. We observed a significantly higher density of CD1

Indexed as

Fluorescent Antibody TechniqueGraft RejectionKidney TransplantationLeukocytes, MononuclearAdultAgedAllograftsFemaleHumansKiller Cells, NaturalMaleMiddle AgedMonocytesRetrospective StudiesT-Lymphocytesantibody-mediated rejection (ABMR)kidney transplantationmultiplex immunofluorescence (OPAL)T-cell-mediated rejection (TCMR)

Identifiers

PMID41373718
PMCPMC12692199

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.