Evidence map›Paper›PMID 41373453›Full record

ArticleInternational journal of molecular sciences2025

A Novel Chimeric Molecule of Heparanase and Ig-Fc Enables Histochemical and Cytochemical Detection of

Jia Shi, Momoko Nakamura, Ryoya Baba, Sojiro Arakawa, Arisa Yamaguchi, Tomonori Hariya, Rin Suzuki, Yu Inazuki, Katsuhiko Takahashi, Makoto Tsuiji and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Jia ShiDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Momoko NakamuraDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Ryoya BabaDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Sojiro ArakawaDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Arisa YamaguchiDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Tomonori HariyaDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Rin SuzukiDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Yu InazukiDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Katsuhiko TakahashiDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.ORCID 0000-0002-8903-2548
Makoto TsuijiDepartment of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.ORCID 0000-0002-5607-4880
Teruaki OkuDepartment of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.ORCID 0000-0001-7360-6642
Mayumi KomineDepartment of Dermatology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke 329-0498, Japan.ORCID 0000-0002-1086-1803
Momo ShimekakeDepartment of Clinical and Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Sciences, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
Kyohei HigashiDepartment of Clinical and Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Sciences, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.ORCID 0000-0002-2622-4825
Masao NakamuraDepartment of Peptidomics, Sasaki Institute, 2-2, Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan.ORCID 0000-0001-6171-3942
Kazuki SasakiDepartment of Peptidomics, Sasaki Institute, 2-2, Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan.ORCID 0009-0006-9126-0351
Motowo NakajimaSBI Pharmaceuticals Co., Ltd., 1-6-1, Roppongi, Minato-ku, Tokyo 106-6019, Japan.ORCID 0000-0001-7687-6365
Tatsuro IrimuraDivision of Glycobiologics, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.ORCID 0000-0002-4709-3131
Nobuaki HigashiDepartment of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.ORCID 0000-0001-6803-6147

Funding

Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology 22K06587Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology 25K09962the joint research program of the J-GlycoNet cooperative network (MEXT) 25C014
6 · The paper itself

Abstract

A chimeric protein of heparanase and Ig-Fc was designed as a novel tool to expand the detection of structurally heterogeneous heparan sulfate (HS) and related glycosaminoglycans. The whole mouse heparanase gene was combined with the gene segment encoding the mouse IgG1 hinge-Fc domain. A point mutation E335A was inserted to disable putative HS degradation activity. Chimeric proteins consisted of the latent form of the enzyme devoid of HS degradation activity. The chimeric proteins bound to heparin,

Indexed as

GlucuronidaseHeparan SulfateRecombinant Fusion ProteinsA549 CellsAnimalsExostosin 1HeparanaseHumansImmunoglobulin GMiceN-AcetylglucosaminyltransferasesSyndecan-1U937 CellsExostosin 1GlucuronidaseHeparanaseHeparan SulfateImmunoglobulin GN-AcetylglucosaminyltransferasesRecombinant Fusion ProteinsSyndecan-1atopic dermatitis skinFc-chimeric moleculesheparanaseheparan sulfateheparininflammatory leukocytesO-sulfationsyndecan

Identifiers

PMID41373453
PMCPMC12691714

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.