False positive results obtained with latex agglutination identification of Staphylococcus aureus caused by Macrococcus caseolyticus and Mammaliicoccus fleurettii.

Magdalena Kizerwetter-Świda, Monika Garbowska, Ilona Stefańska, Joanna Pławińska-Czarnak

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Magdalena Kizerwetter-ŚwidaDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego Str. 8, 02-786, Warsaw, Poland. magdalena_kizerwetter_swida@sggw.edu.pl.

Monika GarbowskaDepartment of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska Str. 166, 02-787, Warsaw, Poland.

Ilona StefańskaDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego Str. 8, 02-786, Warsaw, Poland.

Joanna Pławińska-CzarnakDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego Str. 8, 02-786, Warsaw, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reliable and rapid identification of Staphylococcus aureus constitutes a critical issue in food safety. A total of 180 meat samples were analysed, and 30 isolates that phenotypically matched S. aureus were obtained. All 30 isolates generated positive results in agglutination with the SlidexStaph test. The results of agglutination were verified with the matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) method, S. aureus-specific PCR and sequencing of the 16 S rRNA genes. Among 30 isolates positive in the SlidexStaph test, 17 were finally identified as S. aureus, eight as Macrococcus caseolyticus, four as Mammaliicoccus fleurettii (previously Staphylococcus fleurettii), and one single isolate as Staphylococcus pseudintermedius. In this study, we have demonstrated for the first time false-positive results of the rapid identification based on S. aureus-specific agglutination obtained for M. fleurettii. Antimicrobial resistance testing revealed a considerable prevalence of multidrug resistance among M. caseolyticus (100%) and M. fleurettii (50%). This study highlights the possibility of obtaining false-positive results in agglutination tests with M. caseolyticus and M. fleurettii. Furthermore, the presence of multidrug-resistant M. caseolyticus and M. fleurettii in meat samples may contribute to the spread of microbial drug resistance and requires further investigation.

Indexed as

EnterococcaceaeStaphylococcus aureusAnimalsAnti-Bacterial AgentsFalse Positive ReactionsLatex Fixation TestsMeatMicrobial Sensitivity TestsRNA, Ribosomal, 16SSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnti-Bacterial AgentsRNA, Ribosomal, 16SAgglutinationIdentificationMacrococcus caseolyticusMammaliicoccus fleurettiiStaphylococcus aureus

Identifiers

PMID41372499

DOI10.1038/s41598-025-31847-9

PMCPMC12808235

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