Evidence map›Paper›PMID 41372396›Full record

ArticleCommunications biology2025

Prospective isolation of mouse and human hematopoietic stem cells using PLXDC2.

Yosuke Tanaka, Yasushi Kubota, Riko Kikuchi, Tomohiro Yabushita, Takaharu Kimura, Ivo Lieberam, Jillian L Barlow, Josh W Bramley, Tsuyoshi Fukushima, Chiaki Sakuma and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Yosuke Tanaka *Division of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. yosuketa@g.ecc.u-tokyo.ac.jp.ORCID http://orcid.org/0000-0002-8538-0916
Yasushi Kubota *Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan. kubota-yasushi@koseikan.jp.ORCID http://orcid.org/0000-0001-7785-1362
Riko KikuchiDepartment of Hematology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Tomohiro YabushitaLaboratory of Stem Cell Regulation, International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan.
Takaharu KimuraDivision of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Ivo LieberamCentre for Gene Therapy and Regenerative Medicine and Centre for Developmental Neurobiology, King's College London, London, UK.ORCID http://orcid.org/0000-0003-3539-3054
Jillian L BarlowDepartment of Biology, York Biomedical Research Institute, University of York, York, UK.
Josh W BramleyDepartment of Biology, York Biomedical Research Institute, University of York, York, UK.
Tsuyoshi FukushimaDivision of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Chiaki SakumaKyokuto Pharmaceutical Industrial Co., Ltd, Tokyo, Japan.
Takashi ShibataKyokuto Pharmaceutical Industrial Co., Ltd, Tokyo, Japan.
Masataka NakagawaKyokuto Pharmaceutical Industrial Co., Ltd, Tokyo, Japan.
Yasunori KurosawaKyokuto Pharmaceutical Industrial Co., Ltd, Tokyo, Japan.
Toshiaki MaruyamaAbwiz Bio, Inc., San Diego, CA, USA.ORCID http://orcid.org/0000-0001-8746-3670
C J OkumuraAbwiz Bio, Inc., San Diego, CA, USA.ORCID http://orcid.org/0000-0001-9945-5723
Yuichiro ArimaDepartment of Anatomy, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
Yoshinori SatoDepartment of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan.
Yasuo OnoDepartment of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan.
Teruo AkutaKyokuto Pharmaceutical Industrial Co., Ltd, Tokyo, Japan.ORCID http://orcid.org/0000-0002-0669-4379
Hidenobu MizunoLaboratory of Multi-dimensional imaging, International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan.ORCID http://orcid.org/0000-0001-6262-7106
David G KentDepartment of Biology, York Biomedical Research Institute, University of York, York, UK.ORCID http://orcid.org/0000-0001-7871-8811
Thomas M JessellHoward Hughes Medical Institute, Zuckerman Institute, Depts. of Neuroscience, and Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Susumu GoyamaDivision of Molecular Oncology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0002-9339-1826
Hidekazu NishikiiDepartment of Hematology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Shinya KimuraDivision of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan.ORCID http://orcid.org/0000-0003-1717-6208
Satoshi YamazakiDivision of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Toshio SudaLaboratory of Stem Cell Regulation, International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan.ORCID http://orcid.org/0000-0001-7540-1771
Toshio KitamuraDivision of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0001-7855-1767

Funding

Japan Agency for Medical Research and Development (AMED) 20317838MEXT | Japan Society for the Promotion of Science (JSPS) 17K19645MEXT | Japan Society for the Promotion of Science (JSPS) 19K22380
6 · The paper itself

Abstract

Numerous strategies exist to isolate hematopoietic stem cells (HSCs) using complex combinations of markers and flow cytometry. However, robust identification of HSCs using imaging techniques is substantially more challenging which has prompted the recent development of HSC reporter mice. To date, very few molecules used in these reporters have been useful for human HSC identification. Here we report that PLXDC2 is a useful marker for both mouse and human cord blood HSCs. Using a green fluorescent protein (GFP) knock-in at the Plxdc2 locus in mice (hereafter denoted as Plxdc2-GFP), we showed that Plxdc2-GFP is highly expressed in HSCs with 1 in 2.8 Plxdc2-GFP

Indexed as

Cell SeparationHematopoietic Stem CellsAnimalsFetal BloodFlow CytometryGene Knock-In TechniquesGreen Fluorescent ProteinsHematopoietic Stem Cell TransplantationHumansMiceGreen Fluorescent Proteins

Identifiers

PMID41372396
PMCPMC12770566

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.