ArticleScientific reports2025
Overexpression of milk thistle SOD gene enhances drought tolerance in tobacco by improving photosynthesis and photoprotection.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superoxide dismutase (SOD) is involved in the plant’s primary defense mechanism against the adverse effects of reactive oxygen species (ROS), maintaining ROS homeostasis within cells. Therefore, it is essential to investigate and compare the stress tolerance mechanism in both tolerant and sensitive plants. This study investigates the drought stress tolerance of Tobacco following transferring of a SOD gene obtained from a drought-tolerant plant. The SOD gene was isolated from the Milk thistle (Silybum marianum L.) plant (SmSOD), cloned into the pBI121 (Binary Agrobacterium plasmid) expression vector, and subsequently transformed into Agrobacterium tumefaciens. Pre-cultivated Tobacco (Nicotiana tabacum L.) plants were inoculated with the recombinant bacteria. Following validation of transgene integration via PCR, plants subjected to drought stress corresponding to ~ 50% field capacity for 5 days. Results demonstrated that the expression of SOD and its enzyme activity in drought-imposed transgenic plants were respectively 5 and 1.8 times higher than its expression and activity in wild-type plants. Imaging of chlorophyll fluorescence showed that drought-imposed transgenic plants had a higher maximum quantum yield of photosystem II (Fv/Fm) and Non-photochemical quenching (NPQ) compared with wild-type plants. Stomatal density in drought-imposed plants was lower than in control plants, consistent across both transgenic and wild-type groups. Stomatal width decreased under stress in both plant types, with transgenic plants showing smaller width than wild-type plants. Stomatal length showed no significant differences between transgenic and wild-type plants or between drought-stressed and control conditions. In conclusion, SmSOD gene transfer from the Milk thistle to Tobacco plants increased the drought tolerance of the transgenic plants; this shed light on the path toward reaching drought-tolerant crops. These findings highlight the potential of SmSOD as a key gene for improving drought resistance in crops, offering promising applications in developing drought-tolerant crops for agriculture in water-scarce regions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.