ArticleScientific reports2025
Characterization of myogenesis in European sea bass (Dicentrarchus labrax) using primary white muscle cell cultures.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Derivation and characterization of an embryonic‑derived muscle progenitor cell line from Atlantic salmon (Salmo salar).In vitro cellular & developmental biology. Animal · 2026Article
- Establishment, characterization and myogenic capacity of a muscle-derived cell line from Korean rockfish (Sebastes schlegelii).Fish physiology and biochemistry · 2026Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
This study presents the first characterization of a primary cell culture from white skeletal muscle of European sea bass (Dicentrarchus labrax). Using immunofluorescence and gene expression analyses over 12 days, cell activation, proliferation, differentiation, fusion, and maturation phases were described. During culture development, myogenic regulatory factors (myf5, myod1, myod2, myog, mrf4) were sequentially expressed. Proliferation peaked at days 4-6, with high Pcna and Myod immunodetection and gene expression of pax7, c-met, and pcna. Early downregulation of cell cycle regulators, cdkn1a and cdkn1cb, and mstnb may have contributed to proliferation, while cdkn1bb progressively increased, likely to promote differentiation. The Gh/Igf axis showed differential regulation, igf-1 decreasing early and igf-2, igf-1ra, igf-1rb, and igfbp-1a gradually rising. Differentiation, myotube formation, and maturation were marked by higher Myhc staining, sarcomere development, and upregulation of cdh15, cav3, mef2, mymk, mymx, myhcb, and wnt4. Anabolic (akt2, mtor, eif4ebp1) and proteolytic-related genes (foxo1a, murf1, mafbx, capn1, capn3b, atg12, map1lc3b) increased in later stages. Comparison with other vertebrates revealed both conserved and species-specific regulatory mechanisms of myogenesis. These findings provide a comprehensive molecular framework of skeletal muscle development in European sea bass and establish a valuable in vitro model for studying fish muscle biology and potential aquaculture and biotechnology applications.
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