Evidence map›Paper›PMID 41370351›Full record

ArticlePloS one2025

How much genetic information in RNA form can be protected by a CCMV virus-like particle?

Ana Luisa Duran Meza, Sophie-Christine Porak, Abigail Chapman, Charles M Knobler, William M Gelbart

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ana Luisa Duran MezaDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, United States of America.
Sophie-Christine PorakDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, United States of America.ORCID https://orcid.org/0009-0002-1262-2205
Abigail ChapmanDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, United States of America.ORCID https://orcid.org/0000-0001-7715-4129
Charles M KnoblerDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, United States of America.
William M GelbartDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, United States of America.ORCID https://orcid.org/0000-0003-2125-4256

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The capsid protein of cowpea chlorotic mottle virus (CCMV) has been used extensively for in vitro packaging of heterologous RNA. The associated virus-like particles (VLPs) are spherical with a 3nm-thick/28nm-diameter protein shell and are therefore limited in the amount of RNA they can package. As shown in earlier work, when RNA lengths are longer than ~3500nt the RNA is no longer self-assembled exclusively into a single VLP. Rather, it is shared by two or more 28nm-diameter capsids in the form of doublets, triplets, and higher-order multiplets, with the RNA threaded through the naturally- occurring ~1.5nm-diameter holes in the 180-subunit/icosahedrally-symmetric protein shells. Consistent with this fact we find in the present work that 3500nt is the maximum length of packaged RNA that is fully protected under strong RNase digestion conditions.

Indexed as

BromovirusRNA, ViralVirionCapsidCapsid ProteinsVirus AssemblyCapsid ProteinsRNA, Viral

Identifiers

PMID41370351
PMCPMC12694869

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.