Evidence map›Paper›PMID 41369802›Full record

ArticleGenetica2025

Genome-wide exploration of 2OGD superfamily in Raphanus sativus: unveiling two ANS genes as key players in anthocyanin biosynthesis.

Tongjin Liu, Yangyang Xu, Qunxiang Cui, Lu Zhou, Changyi Wang, Qiuyan Ban

Abstract read
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In one paragraph

Article in Genetica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tongjin Liu *College of Horticulture, Jinling Institute of Technology, Nanjing, 210038, China.
Yangyang Xu *College of Horticulture, Jinling Institute of Technology, Nanjing, 210038, China.
Qunxiang CuiCollege of Horticulture, Jinling Institute of Technology, Nanjing, 210038, China.
Lu ZhouCollege of Horticulture, Jinling Institute of Technology, Nanjing, 210038, China.
Changyi WangCollege of Horticulture, Jinling Institute of Technology, Nanjing, 210038, China.
Qiuyan BanCollege of Horticulture, Jinling Institute of Technology, Nanjing, 210038, China. banqy2019@jit.edu.cn.

Funding

National Natural Science Foundation of China 31801858Research Foundation for Talented Scholars of Jinling Institute of Technology jit-b-202009Research Foundation Incubation Project of Jinling Institute of Technology jit-fhxm-202113
6 · The paper itself

Abstract

The 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily is critical for plant primary and secondary metabolism, but its evolutionary dynamics in radish (Raphanus sativus L.) remain uncharacterized. This study identified 165 radish 2OGD members with distinct physicochemical properties, including amino acid lengths ranging from 122 to 546, molecular weights from 13.1 to 60.1 kDa, and predominant subcellular localizations in the cytoplasm, nucleus, and chloroplast. Phylogenetic analysis clustered these 2OGD genes into 20 clades, functionally categorized into groups involved in hormone metabolism, flavonoid biosynthesis, and specialized metabolite synthesis. Chromosomal localization revealed uneven distribution across 9 chromosomes, with 89 pairs of segmental duplicates and significant syntenic relationships with Arabidopsis 2OGD genes, indicating expansion via gene duplication. Two ANS homologs, RsANS1 and RsANS2, in the LDOX clade were highly expressed in red radish taproots, their overexpression in Arabidopsis enhanced anthocyanin content.​ This study clarifies the evolutionary dynamics of the radish 2OGD superfamily, confirms the role RsANS1 and RsANS2 in anthocyanin biosynthesis, and lays a foundation for investigating the functions of 2OGD genes in regulating metabolite diversification and phenotypic development in radish.

Indexed as

AnthocyaninsDioxygenasesPlant ProteinsRaphanusArabidopsisEvolution, MolecularGene Expression Regulation, PlantGenome, PlantMultigene FamilyPhylogenyAnthocyaninsDioxygenasesPlant Proteins2-oxoglutarate-dependent dioxygenase superfamilyAnthocyanidin synthase (ANS)AnthocyaninBiological functionRadish

Identifiers

PMID41369802

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.