ArticlePlant cell reports2025
Establishment of a TRV-based virus-induced gene silencing system using the vacuum infiltration in Tartary buckwheat.
Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
key messageA TRV-based and bud-vacuum-infiltration-mediated VIGS system achieves an effective gene silencing across multiple varieties under defined optimal conditions in Tartary buckwheat (TB). TB is a valuable grain rich in bioactive flavonoids, but the absence of a genetic transformation system has hindered in vivo functional verification of its genes. Virus-Induced Gene Silencing (VIGS) offers a rapid and efficient alternative for gene studies. This study established and optimized a VIGS system for TB using the phytoene desaturase gene (FtPDS) as a reporter, whose silencing causes photobleaching (albinism). Bioinformatic analysis revealed that FtPDS has a 6,127 bp genomic sequence and a 1,713 bp cDNA, having high similarity to rice phytoene desaturase (OsPDS). We compared two infiltration methods for VIGS delivery: leaf injection into TB cotyledons versus bud vacuum infiltration of germinated seeds, and found the bud vacuum method was more effective, yielding a higher albinism rate (28.9% vs. 12.3%). Using response surface methodology, we determined optimal conditions including germination length at 1.5 cm, the Agrobacterium OD
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