Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis.

Martin Philipp Dieterle, Thorsten Steinberg, Ayman Husari, Pascal Tomakidi

Abstract read

In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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5 · Who and what money

Authors and funding

4 authors.

Martin Philipp DieterleDivision of Oral Biotechnology, Center for Dental Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany.

Thorsten SteinbergDivision of Oral Biotechnology, Center for Dental Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany.ORCID 0000-0003-1924-2694

Ayman HusariDepartment of Orthodontics, Center for Dental Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany.

Pascal TomakidiDivision of Oral Biotechnology, Center for Dental Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early molecular events underlying ethanol-induced oral squamous cell carcinoma development remain insufficiently understood, primarily due to a lack of suitable in vitro systems that recapitulate the initial stages of premalignant transformation. Therefore, a cell culture model of human gingival keratinocytes representing progressive stages of early ethanol-induced cell transformation was established and comprehensively characterized. The three cell lines, named "gingival keratinocytes" (GK), "epithelioid" (EPI) and "fibroblastoid" (FIB), and their derivatives were analyzed by morphological, cell biological and biochemical methods, with an emphasis on epithelial-to-mesenchymal transition (EMT)-related signaling pathways. All cell lines were non-tumorigenic in vitro. Chronic ethanol exposure induced distinct morphological and molecular alterations that capture early premalignant changes in vitro. This includes reduced

Indexed as

CarcinogenesisEpithelial-Mesenchymal TransitionEthanolGingivaKeratinocytesMouth NeoplasmsPrecancerous ConditionsCadherinsCell LineCell Transformation, NeoplasticHumansModels, BiologicalReactive Oxygen SpeciesSignal TransductionVimentinCadherinsEthanolReactive Oxygen SpeciesVimentinCadherinscarcinogenesisepithelial–mesenchymal transitionethanolhuman papillomavirussquamous cell carcinoma of head and neckVimentin

Identifiers

PMID41369376

DOI10.3390/cells14231887

PMCPMC12691217

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.