Evidence map›Paper›PMID 41366768›Full record

ArticleBMC veterinary research2025

Identification of two conserved B-cell epitopes targeting NSP4 of porcine reproductive and respiratory syndrome virus.

Xu-Yan Xiang, Liang Meng, Xin-Yu Qi, Xin-Qi Shi, Xuehui Cai, Tong-Qing An, Hai-Wei Wang

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Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Xu-Yan Xiang *State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China.
Liang Meng *State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China.
Xin-Yu QiState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China.
Xin-Qi ShiState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China.
Xuehui CaiState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China.
Tong-Qing AnState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China.
Hai-Wei WangState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin, 150069, China. wanghaiwei@caas.cn.

Funding

the National Key Research and Development Program of China 2022YFD1800305-02
6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen that causes significant economic losses in the global swine industry. Its non-structural protein NSP4 is a 3 C-like serine protease (3CLSP), which plays a crucial role in viral polyprotein processing and replication. Here, we prepared two monoclonal antibodies (mAbs) against NSP4, which were designated as 7H10 and 1A2. To indentify the epitopes recognized by these mAbs, the NSP4 protein truncations were performed. The results showed that the minimal epitopes recognized by mAb 7H10 and mAb 1A2 were 122 VITEAGEL 129 and 189 VPSDLCALLA 198, respectively. Alanine scanning analysis indicated that Ile123, Thr124, Gly127, Glu128, and Leu129 are critical for mAb 7H10 recognition, while Val189, Pro190, Ser191, Asp192, and Leu193 are critical for mAb 1A2 recognition. Moreover, these epitopes are highly conserved among PRRSV-2 strains. These findings provide a foundation for further research into the function of NSP4 and the pathogenic mechanisms of PRRSV.

Indexed as

Epitopes, B-LymphocytePorcine respiratory and reproductive syndrome virusViral Nonstructural ProteinsAmino Acid SequenceAnimalsAntibodies, MonoclonalAntibodies, ViralSwineAntibodies, MonoclonalAntibodies, ViralEpitopes, B-LymphocyteViral Nonstructural ProteinsEpitopeMonoclonal antibody (mAb)Nonstructural protein 4 (NSP4)Porcine reproductive and respiratory syndrome virus

Identifiers

PMID41366768
PMCPMC12817708

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