ArticlePlant methods2025
Impedance flow cytometry for rapid quality assessment of protoplast cultures.
Article in Plant methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
backgroundProtoplasts, which are plant cells devoid of cell walls, are valuable tools in plant biotechnology. However, they are highly sensitive to mechanical and osmotic stress during isolation and early culture, often leading to significant loss of viability. Reliable and efficient methods for monitoring protoplast quality are essential for downstream applications.
resultsWe applied impedance flow cytometry to assess the viability, cell size, and early division of freshly isolated protoplasts from Arabidopsis thaliana, Brassica napus, and Beta vulgaris. This label-free technique enables fast, objective, and high-throughput assessment of individual protoplasts, allowing reliable monitoring of viability and early division in large populations. Importantly, IFC-derived viability metrics strongly correlated with microcallus formation, demonstrating their predictive value for culture competence.
conclusionsImpedance flow cytometry provides a robust, efficient and reproducible method for characterizing protoplast cultures. It enables rapid assessment of viability and growth potential, supporting quality control and optimization in plant cell culture workflows.
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