Evidence map›Paper›PMID 41366406›Full record

ArticleBMC veterinary research2025

Distribution of Staphylococcus aureus drug resistance genes, biofilm formation and cell wall characteristics in dairy cattle from dairy farms in Northeast China.

Wei Wu, Hongzhe Zhao, Shize Hao, Guiwei Li, Xihuai Xue, Jiaxuan Li, Wen Cui, Yanping Jiang, Yingying Ma, Xinyuan Qiao

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Wei Wu *Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Hongzhe Zhao *Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Shize HaoHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Guiwei LiInstitute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin, 150030, China.
Xihuai XueDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3B2, Canada.
Jiaxuan LiHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Wen CuiHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Yanping JiangHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Yingying MaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. myy930823@163.com.
Xinyuan QiaoHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. qiaoxinyuan@126.com.

Funding

Key Research and Development Program of Heilongjiang Province GA21B004
6 · The paper itself

Abstract

The spread of drug-resistant Staphylococcus aureus(S.aureus) in cattle herds not only threatens livestock production but also becomes an important route for the transmission of drug-resistant bacteria to humans, posing significant public health risks and epidemiological significance. In this study, a total of 282 clinical samples were collected form cattle farms in Heilongjiang, Jilin, and Inner Mongolia, and isolation of S. aureus from 62 samples. Through spa typing and disk diffusion assays of 62 isolates, the predominant spa type was identified as t034. Notably, 54 isolates exhibited multidrug resistance, with a penicillin resistance rate as high as 87.1%, and 34 isolates were identified as methicillin-resistant S. aureus (MRSA). Among the 11 resistance genes tested, the detection rate of tetM gene was 100%. The Congo red plates and crystal violet staining assay showed that the isolated strains all produced biofilms, with no significant changes in the cell wall. In addition, icaA, SarA, and CidA genes identified as critical regulators of biofilm formation. Results from this study suggest that the feces and milk of dairy cows in Northeast China were contaminated with S. aureus including MRSA strains, and these strains carry a variety of resistance genes and have the ability to form biofilms, showing a resistance phenotype to a variety of antimicrobial agents.

Indexed as

BiofilmsCattle DiseasesDrug Resistance, BacterialStaphylococcal InfectionsStaphylococcus aureusAnimalsAnti-Bacterial AgentsCattleCell WallChinaDairyingDrug Resistance, Multiple, BacterialFemaleGenes, BacterialMethicillin-Resistant Staphylococcus aureusMicrobial Sensitivity TestsAnti-Bacterial AgentsAntibiotic resistanceBiofilmBovine-derived S. aureusResistance genes

Identifiers

PMID41366406
PMCPMC12805691

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.