ArticleBMC microbiology2025
Characterization and modification of phenolic acid decarboxylase from marine bacteria for the synthesis of 4-vinyl phenol derivatives.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Screening of a 4-Ethylguaiacol-ProducingMicroorganisms · 2026Article
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10 authors.
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Abstract
backgroundThe 4-vinyl derivatives of various hydroxycinnamic acids are highly valued for their substantial application in the food and other industries. With growing demand for natural spice flavors, the nonoxidative decarboxylation of phenolic acid decarboxylase has attracted significant attention as a method for biosynthesizing 4-vinyl derivatives. This approach offers several advantages, such as mild reaction conditions, excellent substrate affinity, and high reaction efficiency.
resultsIn this study, we successfully screened a new strain, Bacillus sp. QCS58, from among 40 marine bacteria for the first time. This strain harbors the phenolic acid decarboxylase gene. Subsequently, we cloned and overexpressed the phenolic acid decarboxylase BaPAD-Q58 from strain QCS58 in Escherichia coli. Enzymatic property assays revealed that BaPAD-Q58 exhibited enhanced stability in organic solvents and displayed optimal catalytic activity at pH 6.0 and 35 °C. To improve the substrate affinity of BaPAD-Q58, we conducted tertiary structure prediction and molecular docking analysis to guide site-directed mutagenesis. Importantly, site-directed mutagenesis significantly improved the binding affinity between the phenolic acid decarboxylase and its substrate, as evidenced by a Km value of 0.51 ± 0.14 mM for the variant BaPAD-Q58
conclusionThe mutant BsPAD-Q58
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