ArticleBiochemistry2026
Long-Patch Base Excision Repair of 5'-DNA-Peptide Cross-Links Derived from Abasic DNA Lesions.
Article in Biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
DNA single-strand breaks (SSBs) containing covalent DNA-protein cross-links at 5'-termini (5'-DPCs) are produced from the C1'-oxidized abasic site, 2-deoxyribonolactone. These adducts need to be removed for SSB repair because 5'-phosphate is required for strand ligation. Prior studies showed that 5'-DPCs can undergo proteolysis by the 26S proteasome. However, how the remaining 5'-DNA-peptide cross-links (5'-DpCs) are removed is unclear. Herein, we found that a chemically synthesized and site-specific 5'-DpC can be repaired by HeLa cell nuclear extracts, and human flap-endonuclease 1 (hFEN1) plays an essential role in the DpC excision. We also synthesized a model 5'-DPC by reductive amination and showed that prior proteolysis of the cross-linked protein by trypsin greatly facilitated the DPC repair in HeLa cell nuclear extracts. Our findings suggest that 5'-DPCs within SSBs can be repaired by proteolysis followed by the long-patch base excision repair pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.