Evidence map›Paper›PMID 41359424›Full record

ArticleCell reports2025

Context-dependent inhibitory roles of RhoA in 3D invasive cell migration within the extracellular matrix.

Wakiko Iwata, Yoshihiro Adachi, Junior J West, Andrew J Ewald, Yi Zheng, Laura D Wood, Robert A Anders, Hiromi Sesaki, Miho Iijima

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wakiko IwataDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Yoshihiro AdachiDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Junior J WestDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Andrew J EwaldDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Giovanis Institute for Translational Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University Whiting School of Engineering, Baltimore, MD, USA.
Yi ZhengDivision of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Laura D WoodDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Robert A AndersDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Hiromi SesakiDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Miho IijimaDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: miijima@jhmi.edu.

Funding

Regulation of Intracellular SignalingR35GM131768 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Miho Iijima · 2019 to 2026
$4.0M
Mapping the single cell state basis of metastasis in space and timeU01CA284090 · NCI · JOHNS HOPKINS UNIVERSITY · PI Andrew Josef Ewald · 2023 to 2026
$2.7M
Mechanism and Function of the Supercomplex KARATE in Insulin SignalingR01DK129699 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI IIJIMA, MIHO · 2022 to 2025
$1.7M
NCI NIH HHS U01 CA284090NIDDK NIH HHS R01 DK129699NIGMS NIH HHS R35 GM131768
6 · The paper itself

Abstract

Cell migration is fundamental to both physiological and pathological processes, including cancer progression. This study investigates the role of the small GTPase RHOA in invasive cell migration within diverse 3D extracellular matrix (ECM) environments using non-cancerous HEK293, pancreatic cancer PANC-1, and breast cancer MDA-MB-231 cells. Spheroid invasion assays showed that RHOA loss enhanced migration in HEK293 and PANC-1 cells cultured in Geltrex but not in type I collagen. In contrast, RHOA deletion had little effect on MDA-MB-231 migration in either ECM. Enhanced migration in RHOA-deficient HEK293 cells required protein phosphatase PTP1B and the small GTPases RAC and CDC42. Unexpectedly, while RHOA knockout increased 3D migration, it reduced pancreatic tumor progression in mice. These findings reveal that RHOA regulates cell invasion in a manner dependent on ECM composition and cellular context, highlighting its complex, context-specific roles and potential as a therapeutic target in cancer.

Indexed as

Cell MovementExtracellular MatrixrhoA GTP-Binding ProteinAnimalscdc42 GTP-Binding ProteinCell Line, TumorFemaleHEK293 CellsHumansMiceNeoplasm InvasivenessPancreatic Neoplasmscdc42 GTP-Binding ProteinrhoA GTP-Binding ProteinRHOA protein, human3D cell migrationcancercancer invasionCP: CancerCP: Cell biologyKPCPDACRHOA

Identifiers

PMID41359424
PMCPMC12823269

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.