Evidence map›Paper›PMID 41358557›Full record

ArticleCytoskeleton (Hoboken, N.J.)2026

The Influence of Drosophila Spire and Myosin V During Mid-Oogenesis Is Independent of Their Direct Interaction.

Joseph Y Ong, Hannah M Bailey, Emma Carley, Michelle Panzica, Merin Rixen, Neil Billington, Rong Liu, Margot E Quinlan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytoskeleton (Hoboken, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Joseph Y OngDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Hannah M BaileyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Emma CarleyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Michelle PanzicaDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Merin RixenDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Neil BillingtonDepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, West Virginia, USA.ORCID https://orcid.org/0000-0003-2306-0228
Rong LiuDepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, West Virginia, USA.
Margot E QuinlanDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.ORCID https://orcid.org/0000-0002-8133-1033

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
Collaboration between actin nucleators - Spire and CappuccinoR01GM096133 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI QUINLAN, MARGOT E · 2011 to 2024
$4.1M
National Heart Lung and Blood Institute L'Enfant Biomedical FellowshipNational Science Foundation DGE-1650604NIGMS NIH HHS R01 GM096133NIGMS NIH HHS R01GM096133NIGMS NIH HHS T32 GM007185NIGMS NIH HHS T32GM007185NIH HHS P40 OD018537
6 · The paper itself

Abstract

Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin-associated proteins like myosins and Rab GTPases. Here, we investigate the interaction between Spire and Myosin V (MyoV) in Drosophila oogenesis. We combine in vitro biochemical assays with immunofluorescence and genetics to probe the interaction and its impact on polarity establishment and the actin mesh that fills the oocyte during mid-oogenesis. Expressed Spire and MyoV constructs colocalize in punctae during mid oogenesis, with considerable enrichment near the oocyte cortex. Purified constructs interact directly in vitro, and we find that Spire can weakly activate MyoV ATPase activity. Cytoplasmic flows, critical for polarity establishment, are faster and more coordinated in the absence of MyoV, although not to the extent of fast streaming. This intermediate streaming has not been observed before. Interestingly, this MyoV-dependent change in ooplasm dynamics is sensitive to Spire levels. Despite this interplay, the actin mesh and polarity establishment appear normal when binding mutants of Spire and MyoV are expressed in the Drosophila germline. These findings suggest that direct interaction between Spire and MyoV is not essential for their primary roles at this stage of development.

Indexed as

Drosophila melanogasterDrosophila ProteinsMicrofilament ProteinsMyosin Type VOogenesisActinsAnimalsDrosophilaFemaleOocytesActinsDrosophila ProteinsMicrofilament ProteinsMyosin Type Vspir protein, DrosophilaactinDrosophilamyosin Voogenesisspire

Identifiers

PMID41358557
PMCPMC13648917

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.