ArticleFrontiers in pharmacology2025
Development and validation of a two-dimensional liquid chromatography method for therapeutic drug monitoring of pyrotinib in human plasma.
Article in Frontiers in pharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Effect of dosing interval on the pharmacokinetic interaction between montmorillonite powder and pyrotinib in rats.Frontiers in pharmacology · 2026Article
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8 authors.
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Abstract
Objective: To develop and validate a method for therapeutic drug monitoring (TDM) of pyrotinib in human plasma using two-dimensional liquid chromatography (2D-LC) system. Method: The plasma samples were pretreated with acetonitrile for protein precipitation. The mobile phase consisted of two parts: a first-dimensional mobile phase (methanol, acetonitrile, and 65 mmol/L ammonium phosphate in a ratio of 1:3:3, V/V/V) and a second-dimensional mobile phase (acetonitrile, isopropanol, and 10 mmol/L ammonium phosphate in a ratio of 16:7:1, V/V/V). The analysis cycle time was completed within 9.50 min. The method was validated for linearity, recovery, precision, accuracy, and stability. Results: Pyrotinib demonstrated excellent linearity within the range of 10.10-810.40 ng/mL with regression equation y = 556.4044× + 462.40 ( Conclusion: The developed 2D-LC analytical method not only demonstrates good precision, accuracy, recovery, and stability, but also is simple, rapid, feasible, and practical for TDM. It can be used for the concentration monitoring of pyrotinib in clinic, providing more scientific evidence for clinical practice.
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