ArticleFrontiers in microbiology2025
Genetically engineered
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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12 authors.
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Abstract
Introduction: Rhamnolipids are valuable biosurfactants, but their large-scale application is limited by high production costs. Waste frying oil (WFO), a low-cost and abundant triglyceride-rich byproduct, offers a sustainable carbon source for rhamnolipid synthesis, though its utilization efficiency by microbes like Methods: This study evaluated the potential of engineered Results: Wild-type PAO1 and PAO1ΔaroA could synthesize rhamnolipids from WFO, while PAO1-RhlAB improved yields vs. PAO1 in WFO. Exogenous lipase addition (PAO1+) achieved 14.0 g/L rhamnolipids at 48 h (vs. 9 g/L for PAO1), and the synergistic PAO1-lipase+ reached 16.0 g/L (vs. 12.8 g/L for PAO1-lipase) at the same time. At 144 h, PAO1-lipase+ had the highest oil degradation rate (34.40%), while PAO1+ maintained a slightly higher yield (20 g/L) than PAO1-lipase+ (19 g/L). Discussion: These findings confirm that lipase regulation (overexpression or exogenous addition) enhances WFO utilization and rhamnolipid production, providing a cost-effective approach for sustainable rhamnolipid biosynthesis from waste lipids.
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