ArticleFrontiers in cellular and infection microbiology2025
Direct detection of Gram-negative bacilli and extended-spectrum β-lactamase producers in positive blood culture specimens using MALDI-TOF MS.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Bloodstream infections caused by antimicrobial-resistant Gram-negative bacilli are often difficult to treat. Here, we investigated the performance of a workflow for direct detection of Gram-negative bacilli and extended-spectrum β-lactamase (ESBL) producers in positive blood culture (PBC) specimens using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Samples were prepared from spiked and clinical PBC specimens using a sample preparation kit, and bacterial identification and cephalosporinase activity assays were performed using MALDI-TOF MS. The results were compared with those obtained using the conventional method, which was performed for colonies as routine microbiological testing. Of the 34 spiked PBC specimens prepared using
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