ArticleFrontiers in cellular and infection microbiology2025
Recirculating glass pipettes constitute a high risk when working with freshly isolated immune cells - the presence of bacterial pyrogenic material.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A number of immune cells are highly sensitive to pyrogenic substances such as bacterial lipopolysaccharides (LPS). It is therefore crucial to ensure that all material coming in contact with these cells are pyrogen free. We here present a comparative analysis of the induction of inflammatory cytokines by freshly isolated human peripheral blood monocytes when handling the cells with recirculating glass pipettes or disposable plastic pipettes. The glass pipettes were sterilized but previously used for multiple projects including work with bacteria. Using these glass pipettes resulted in the same induction of a set of inflammatory cytokines and chemokines as when adding 1 ug of LPS/ml to the culture medium, an exceptionally high level of LPS. The most extreme upregulation was seen for IL-6, which increased in expression by a factor of more than 75 000-fold already by four hours of
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