Evidence map›Paper›PMID 41354805›Full record

ArticleVirology journal2025

Whole genome sequence analysis and characterization of virulent Newcastle disease virus isolates from chicken and pheasants during 2020/21 outbreaks in Nepal.

Meera Prajapati, Aashirbad Pokharel, Shresha Rayamajhi, Madhav Prasad Acharya, Manita Aryal, Suruchi Karn, Joe James, Ashley C Banyard, Craig S Ross, Joshua G Lynton-Jenkins

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Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Meera PrajapatiNational Animal Health Research Center, Nepal Agricultural Research Council, Khumaltar, Lalitpur, Nepal. prajapatimeera@gmail.com.
Aashirbad PokharelNational Animal Health Research Center, Nepal Agricultural Research Council, Khumaltar, Lalitpur, Nepal.
Shresha RayamajhiHimalayan College of Agricultural Sciences and Technology, Kirtipur, Kathmandu, Nepal.
Madhav Prasad AcharyaNational Animal Health Research Center, Nepal Agricultural Research Council, Khumaltar, Lalitpur, Nepal.
Manita AryalCentral Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
Suruchi KarnNational Animal Health Research Center, Nepal Agricultural Research Council, Khumaltar, Lalitpur, Nepal.
Joe JamesDepartment of Virology, Animal and Plant Health Agency (APHA- Weybridge), Woodham Lane, Addlestone, Surrey, KT15 3NB, UK.
Ashley C BanyardDepartment of Virology, Animal and Plant Health Agency (APHA- Weybridge), Woodham Lane, Addlestone, Surrey, KT15 3NB, UK.
Craig S RossDepartment of Virology, Animal and Plant Health Agency (APHA- Weybridge), Woodham Lane, Addlestone, Surrey, KT15 3NB, UK.
Joshua G Lynton-JenkinsDepartment of Virology, Animal and Plant Health Agency (APHA- Weybridge), Woodham Lane, Addlestone, Surrey, KT15 3NB, UK. joshua.lynton-jenkins@apha.gov.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Newcastle Disease (ND) remains a major threat to poultry production worldwide, particularly in regions where it is endemic, like Southern Asia. The disease is caused by virulent forms of avian paramyxovirus-1, commonly termed Newcastle Disease Virus (NDV), a highly contagious virus with significant genetic diversity and evolving pathogenicity. This study aimed to molecularly characterize NDV isolates obtained from chickens and pheasants during the 2020/21 ND outbreaks in Nepal, to understand their genetic makeup, phylogenetic relationships, and implications for control strategies. Necropsy samples, including trachea, liver, intestine, spleen, lungs, heart, and proventriculus were collected from ten birds. Isolates from five clinical samples were typed as NDV by hemagglutination and hemagglutination inhibition (HA/HI) assays and were subjected to whole genome sequencing (WGS). Full genomes of 15,192 nucleotides were recovered from each isolate. Fusion (F) gene sequence analysis revealed the presence of multi-basic cleavage site motif

Indexed as

ChickensDisease OutbreaksGalliformesGenome, ViralNewcastle DiseaseNewcastle disease virusPoultry DiseasesAnimalsGenetic VariationNepalPhylogenyVirulenceWhole Genome SequencingF proteinMolecular epidemiologyNewcastle diseaseWhole genome

Identifiers

PMID41354805
PMCPMC12798023

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.