Evidence map›Paper›PMID 41354719›Full record

ArticleProbiotics and antimicrobial proteins2026

Characterization and Biofungicide Potential of a Novel Antifungal defensin, K4CBP6, from Solanum lycopersicum L.

Rebeka Papp, Péter Poór, Zalán Czékus, Györgyi Váradi, Zoltán Kele, Attila Borics, Gábor Bende, Kata Horváti, Gábor K Tóth, László Galgóczy and 1 more

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Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rebeka PappDepartment of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
Péter PoórDepartment of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0002-4539-6358
Zalán CzékusDepartment of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0001-5862-1350
Györgyi VáradiDepartment of Medical Chemistry, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0001-7907-8908
Zoltán KeleDepartment of Medical Chemistry, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0002-4401-0302
Attila BoricsInstitute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary.ORCID http://orcid.org/0000-0002-6331-3536
Gábor BendeDepartment of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0003-4571-9775
Kata HorvátiInstitute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, Budapest, Hungary.ORCID http://orcid.org/0000-0003-4092-6011
Gábor K TóthDepartment of Medical Chemistry, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0002-3604-4385
László GalgóczyDepartment of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary. galgoczi@bio.u-szeged.hu.ORCID http://orcid.org/0000-0002-6976-8910
Liliána TóthDepartment of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.ORCID http://orcid.org/0000-0003-1400-6174

Funding

Magyar Tudományos Akadémia LP2021-28Nemzeti Kutatási Fejlesztési és Innovációs Hivatal ANN 139484Nemzeti Kutatási Fejlesztési és Innovációs Hivatal FK 134343Nemzeti Kutatási Fejlesztési és Innovációs Hivatal FK 138867Nemzeti Kutatási Fejlesztési és Innovációs Hivatal K 143124Nemzeti Kutatási Fejlesztési és Innovációs Hivatal PD 134284Szegedi Tudományegyetem Open Access Fund 7828
6 · The paper itself

Abstract

The global rise in resistance to chemical fungicides and their strict regulation by the EU, has created an urgent need for alternative antifungal strategies in agriculture. Plant defensins represent promising alternatives owing to their broad-spectrum antifungal activity, structural stability, and low toxicity to mammalian cells and plants. In this study, we identified and characterized a novel antifungal defensin, K4CBP6, from Solanum lycopersicum L., along with its γ-core peptide derivatives, K4CBP6γ1 and K4CBP6γ2, as potential biofungicide agents. Protein database mining revealed a widespread distribution of K4CBP6 homologs within the Solanaceae family. Recombinant K4CBP6 (rK4CBP6) was successfully produced using a Komagataella phaffii-based expression system, while K4CBP6γ1 and K4CBP6γ2 were chemically synthesized. Structural analyses via electrospray ionization mass spectrometry and electronic circular dichroism spectroscopy confirmed a cysteine-stabilized α-helix β-strand folded structure for rK4CBP6. In vitro susceptibility assays demonstrated that both rK4CBP6 and K4CBP6γ2 exhibited antifungal activity against major tomato pathogens, Botrytis cinerea, Cladosporium herbarum, and Fusarium oxysporum with minimum inhibitory concentrations ranging from 12.5 to 25 µg ml

Indexed as

Antifungal AgentsDefensinsPlant ProteinsSolanum lycopersicumAmino Acid SequenceBotrytisFusariumMicrobial Sensitivity TestsPlant DiseasesAntifungal AgentsDefensinsPlant ProteinsAntifungal defensinBiofungicidePlant pathogenic fungusPlant protectionSolanum lycopersicum L.γ-core peptide

Identifiers

PMID41354719
PMCPMC13341736

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.