ArticleParasites & vectors2025
Pedal effect: aerobic and facultative anaerobic Enterobacter hormaechei maintain the homeostasis of the house fly gut microbiota under different oxygen conditions.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
backgroundHouse fly gut microbiota plays a major role in regulating larval development and immune responses. As the house fly gut microbiota is complex with different functions, the role of many gut microbiota in the host remains unknown. The differential oxygen content in the midgut and hindgut of house fly intestines creates distinct gut microenvironments, resulting in variations in the composition of gut microbiota. Research on the distribution of aerobic and facultative anaerobic bacteria in different parts of the intestine-and how they interact with each other-is helpful for exploring the interaction between intestinal bacteria and the host in aerobic and anaerobic states. However, there have been no mechanistic studies on the interactions of gut bacteria in different oxygen microenvironments within the house fly.
methodsIn this study, aerobic Enterobacter hormaechei EhX and facultative anaerobic E. hormaechei EhY were isolated from the guts of house fly larvae. The specific phages, EhXP and EhYP, were isolated against these two bacteria, with phage EhXP and EhYP specifically targeting EhX and EhY, respectively. Furthermore, the role of the aerobic-anaerobic interaction in E. hormaechei during larval development was investigated using phage and bacterial feeding experiments.
resultsThe results showed that the addition of aerobic E. hormaechei EhX or facultative anaerobic E. hormaechei EhY alone to the diet promoted larval growth and phenoloxidase activity. However, in the phage-treated groups, the opposite effect was observed. In contrast, EhY was the dominant bacteria in larval hindgut. Conversely, EhX was the main bacteria in the midgut of larvae. Aerobic/facultative anaerobic gut bacteria competed and cooperated to maintain a stable composition of larval gut microbiota, forming a negative correlational "pedal effect" promoting larval development. Through high-throughput sequencing it was demonstrated that the abundance of beneficial bacteria increased in the bacteria treatment group. The number of harmful bacteria increased in the phage treatment group.
conclusionsThe results revealed the crucial role of aerobic and facultative anaerobic E. hormaechei in the house fly. These results highlight the potential of future applications of multiple phages for the study of insect intestinal flora.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.