Evidence map›Paper›PMID 41353469›Full record

ArticleMolecular neurobiology2025

Alterations in the Expression of Let-7i, miR-21-5p, and miR-30b-5p in Plasma-Derived Extracellular Vesicles as the Possible Prognostic Markers in Central Demyelinating Diseases.

Chutithep Teekaput, Kitti Thiankhaw, Surat Tanprawate, Nattayaporn Apaijai, Kanokphong Suparan, Sirinart Kumfu, Nipon Chattipakorn, Siriporn C Chattipakorn

Abstract read
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Article in Molecular neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chutithep TeekaputDivision of Neurology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Kitti ThiankhawDivision of Neurology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Surat TanprawateDivision of Neurology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Nattayaporn ApaijaiCardiac Electrophysiology Research and Training Center, Faculty of Medicine, Neurophysiology Unit, Chiang Mai University, Chiang Mai, 50200, Thailand.
Kanokphong SuparanCardiac Electrophysiology Research and Training Center, Faculty of Medicine, Neurophysiology Unit, Chiang Mai University, Chiang Mai, 50200, Thailand.
Sirinart KumfuCardiac Electrophysiology Research and Training Center, Faculty of Medicine, Neurophysiology Unit, Chiang Mai University, Chiang Mai, 50200, Thailand.
Nipon ChattipakornCardiac Electrophysiology Research and Training Center, Faculty of Medicine, Neurophysiology Unit, Chiang Mai University, Chiang Mai, 50200, Thailand.
Siriporn C ChattipakornCardiac Electrophysiology Research and Training Center, Faculty of Medicine, Neurophysiology Unit, Chiang Mai University, Chiang Mai, 50200, Thailand. scchattipakorn@gmail.com.

Funding

Chiang Mai Univers CMU Excellent Center awardChiang Mai University Fundamental Fund 2024National Research Council of Thailand N42A660301
6 · The paper itself

Abstract

backgroundNeuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) are central demyelinating diseases with overlapping features but differing immunopathogenesis. Extracellular vesicle (EV)-associated microRNAs (miRNAs) have emerged as potential biomarkers, but their roles in NMOSD remain underexplored. This study aimed to assess EV-miRNA expression changes across NMOSD stages compared to relapsing-remitting MS (RRMS) and healthy controls (HC).

methodsWe enrolled 43 participants, including HC (n = 10), RRMS (n = 10), NMOSD in remission (NMOSD-r, n = 13), and NMOSD exacerbation (NMOSD-ex, n = 10). Plasma-derived EVs from all participants were isolated, and their morphology, size, and concentration were assessed. The expression of let-7i-5p, miR-21-5p, and miR-30b-5p in EV was quantified. EVs of NMOSD-ex patients were re-evaluated at one month after corticosteroid treatment (NMOSD-fu). The correlation between alterations of EV miRNA levels and clinical outcomes was analyzed.

resultsEV concentrations isolated from patients with RRMS, NMOSD-r and NMOSD-ex were significantly lower compared to those of HC, and the smallest of EV sizes was observed in NMOSD-ex group. MiR-30b-5p expression was markedly reduced in NMOSD and RRMS compared to HC, whereas let-7i-5p and miR-21-5p exhibited nonsignificant downward trends. In NMOSD-ex group, miR-30b-5p levels positively correlated with higher glial fibrillary acidic protein (GFAP) and greater disability. Following corticosteroid treatment, increased expression of miR-30b-5p was individually associated with motor function recovery, although the degree of visual improvement was comparatively limited.

conclusionPlasma-derived EV-associated miR-30b-5p may reflect a protective role in response to astrocytic injury and serve as a valuable biomarker for disease activity and treatment response in NMOSD.

Indexed as

Extracellular VesiclesMicroRNAsMultiple Sclerosis, Relapsing-RemittingNeuromyelitis OpticaAdrenal Cortex HormonesAdultBiomarkersDiagnosis, DifferentialFemaleHumansMaleMiddle AgedAdrenal Cortex HormonesBiomarkersMicroRNAsMIRN21 microRNA, humanMIRN30b microRNA, humanmirnlet7 microRNA, humanClinical outcomeExtracellular vesiclesMicroRNAMultiple sclerosisNeuromyelitis optica spectrum disorderTreatment

Identifiers

PMID41353469

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.