Evidence map›Paper›PMID 41353364›Full record

ArticleBMC genomic data2025

Draft genomes of two Streptococcus agalactiae from bovine mastitis in the North-West Province, South Africa.

Kgaugelo Edward Lekota, Lydia Cecilia Sizana, Ntelekwane George Khasapane

Abstract read
In one paragraph

Article in BMC genomic data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kgaugelo Edward LekotaUnit for Environmental Sciences and Management, North-West University, Potchefstroom, 2531, South Africa. 37747959@nwu.ac.za.
Lydia Cecilia SizanaUnit for Environmental Sciences and Management, North-West University, Potchefstroom, 2531, South Africa.
Ntelekwane George KhasapaneDepartment of Life Sciences, Centre for Applied Food Sustainability and Biotechnology, Central University of Technology, Bloemfontein, 9300, South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThis study presents the whole-genome sequence of Streptococcus agalactiae 40LHNT and 10RH2NT, isolated from bovine milk with subclinical mastitis in the North-West Province of South Africa. The objective was to characterize the genomes to understand the antibiotic resistance and virulent potential of the isolates. DATA DESCRIPTION: Genomic DNA of S. agalactiae strains was extracted and sequenced using the Oxford Nanopore MiniON platform. De novo assembly was carried out using Flye v. v2.9, resulting in an average draft genome size of 2.25 Mb for both strains, assembled into 4 contigs for strain 40LHNT and 91 contigs for strain 10RH2NT with an average GC content of 35.5% for both strains. Genome quality assessment revealed an average completeness of 92.64% and a contamination level of 1.6%. For both strains, the closest reference type strain was S. agalactiae NCTC8181 (accession GCA_900458965.1), showing an average nucleotide identity of 97%, thereby confirming species-level identity and close genetic relatedness. Annotation using the NCBI Prokaryotic Genome Annotation Pipeline identified 2,067 and 1977 protein-coding genes for strains 40LHNT and 10RH2NT, respectively..

Indexed as

Genome, BacterialMastitis, BovineStreptococcal InfectionsStreptococcus agalactiaeAnimalsCattleFemalePhylogenySouth AfricaWhole Genome SequencingAntimicrobial resistanceBovine mastitisStreptococcus agalactiaeVirulence factorsWhole genome sequencing

Identifiers

PMID41353364
PMCPMC12797599

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.