Evidence map›Paper›PMID 41353339›Full record

ArticlePlant methods2025

Effect of hormone concentration on callus induction and plant regeneration induced from daylily (Hemerocallis fulva) anther.

Wei Li, Chongcheng Yang, Lixin Huang, Weihao Wu, Qiaoru Tan, Shaoxia Yang, Feng Feng

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In one paragraph

Article in Plant methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Wei Li *College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Chongcheng Yang *College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Lixin HuangCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Weihao WuCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Qiaoru TanCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Shaoxia YangCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China. yangxx@gdou.edu.cn.
Feng FengCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China. ff@gdou.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn modern plant breeding, anther culture is an important biotechnological tool that shortens the breeding cycle and improves efficiency, thereby playing a crucial role in genetic improvement and cultivar development. To date, there have been few studies on anther culture in daylily (Hemerocallis spp.). The present study aimed to investigate the effects of the microspore developmental stage, low-temperature pretreatment duration, and phytohormone combinations on callus induction and plant regeneration from daylily anthers.

resultsWe first studied the morphological characteristics of flower buds and anthers at different microspore developmental stages, and then used anthers from different developmental stages for callus induction. The results showed that microspores at the late uninucleate stage were optimal for callus induction. Low-temperature pretreatment at 4°C for 24 h could effectively promote the formation of callus tissue in daylily anthers. In the (L9(3

conclusionThis study optimized the anther culture technique for daylily and proposed a comprehensive anther culture method for callus induction and plant regeneration. For the first time, plant regeneration was achieved via anther culture in daylily, providing relevant theoretical and technical support for genetic research and daylily breeding.

Indexed as

Anther cultureHemerocallis fulvaHormone concentrationsLow-temperature pre-treatmentMicrospore development stagePloidy identification

Identifiers

PMID41353339
PMCPMC12701605

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