Evidence map›Paper›PMID 41353258›Full record

ArticleScientific reports2025

SOSHI-seq: a high-throughput screening assay to test the functionality of putative response elements for nuclear hormone receptors.

Shijia Wu, Romain Guyot, Yanis Zekri, Wenzheng Jiang, Frédéric Flamant

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shijia WuEcole Normale Supérieure de Lyon, INRAE, CNRS, Institut de Génomique Fonctionnelle de Lyon, 69364, Lyon Cedex07, France.
Romain GuyotEcole Normale Supérieure de Lyon, INRAE, CNRS, Institut de Génomique Fonctionnelle de Lyon, 69364, Lyon Cedex07, France.
Yanis ZekriDivisions of Oncogenomics, The Netherlands Cancer Institute (NKI), Plesmanlaan, 121 1066CX, Amsterdam, Netherlands.
Wenzheng JiangShanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, China.
Frédéric FlamantEcole Normale Supérieure de Lyon, INRAE, CNRS, Institut de Génomique Fonctionnelle de Lyon, 69364, Lyon Cedex07, France. Frederic.flamant@ens-lyon.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a cheap, rapid, and versatile assay called SOSHI-seq (Screening of Self-transcribed Hormone Inducible response elements coupled to sequencing). The protocol is an adaptation of the STARR-seq method, which allows testing the capacity of thousands of synthetic DNA sequences to act as hormone-response elements. Using the nuclear receptors of thyroid hormone as an example, we show that SOSHI-seq is a suitable complement to ChIP-Seq analysis to identify at genome-wide scale the functional response elements occupied by nuclear receptors in chromatin.

Indexed as

High-Throughput Nucleotide SequencingHigh-Throughput Screening AssaysReceptors, Cytoplasmic and NuclearResponse ElementsChromatinHumansReceptors, Thyroid HormoneChromatinReceptors, Cytoplasmic and NuclearReceptors, Thyroid HormoneDeep sequencingGene regulationNuclear receptorsThyroid hormone

Identifiers

PMID41353258
PMCPMC12775020

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.