Evidence map›Paper›PMID 41351054›Full record

ArticleParasites & vectors2025

Pyrethroid insecticide resistance of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) across the Hawaiian Islands.

Sangwoo Seok, Miles T McCollum, Christopher M Jacobsen, Omar S Akbari, Derrick K Mathias, Yoosook Lee

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PubMed Full text at PMC Publisher
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Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Sangwoo SeokFlorida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Florida, USA.
Miles T McCollumFlorida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Florida, USA.
Christopher M JacobsenHawaii State Department of Health, Hilo, HI, USA.
Omar S AkbariSchool of Biological Science, Department of Cell and Developmental Biology, University of California, San Diego, CA, USA.
Derrick K MathiasFlorida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Florida, USA.
Yoosook LeeFlorida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Florida, USA. yoosook.lee@ufl.edu.

Funding

Dispersal biology of invasive Aedes aegypti mosquito populationsR35GM156217 · NIGMS · UNIVERSITY OF FLORIDA · PI Yoosook Lee · 2025 to 2026
$658k
National Institute of Food and Agriculture 1025565NIGMS NIH HHS R35 GM156217NIH HHS R35GM156217U.S. Environmental Protection Agency 84020401
6 · The paper itself

Abstract

backgroundAedes albopictus (Skuse, 1894) and Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae) are invasive species in the Hawaiian Islands as well as other islands of the Pacific and serve as the primary vectors of arboviruses such as dengue virus. Despite its significance to public health, data on their insecticide resistance remains limited. Knowledge of the level of insecticide resistance is critical in developing effective mosquito control strategies, especially when an arboviral disease outbreak occurs.

methodsThis study investigated the phenotypic and genotypic resistance of Hawaiian Ae. albopictus and Ae. aegypti to permethrin, one of the commonly used pyrethroids. Genomic sequences of 93 Ae. albopictus samples from four Hawaiian Islands (Kaua'i, O'ahu, Maui, and the Island of Hawai'i) were screened for non-synonymous mutations in the voltage-gated sodium channel (VGSC) gene (chromosome 3: 345,142,499-345,663,668). Phenotypic resistance to permethrin was assessed using a modified Centers for Disease Control and Prevention (CDC) bottle bioassay on Ae. albopictus and Ae. aegypti collected from two socio-environmentally distinct sites on the Island of Hawai'i.

resultsAmong 4101 single-nucleotide polymorphisms (SNPs) identified in the VGSC region of Ae. albopictus genomes from four Hawaiian Islands, 44 were classified as synonymous in the coding DNA sequence (CDS) region. No non-synonymous mutations were found in the CDS region, suggesting an absence of genotypic resistance to pyrethroids in these populations. In phenotypic assays, all individuals from the two Ae. aegypti populations and the Ae. albopictus population from Miloli'i were completely knocked down within 10 min of permethrin exposure, indicating that both species remain phenotypically susceptible to permethrin. However, knockdown rates of less than 97% observed in the Ae. albopictus population from Kawaihae suggest early signs of the development of phenotypic resistance.

conclusionsThis study is the first to report the phenotypic insecticide resistance profile of Hawaiian Aedes mosquitoes. Hawaiian populations of Ae. albopictus and Ae. aegypti remain susceptible or are in the stage of developing resistance to pyrethroids, as demonstrated by the absence of VGSC mutations and high knockdown rates in permethrin bioassays. While no genotypic and phenotypic resistance was detected in these two Aedes species, monitoring for resistance in other mosquito species and through alternative mechanisms is needed.

Indexed as

AedesInsecticide ResistanceInsecticidesMosquito VectorsPyrethrinsAnimalsFemaleGenotypeHawaiiMutationPermethrinPhenotypeVoltage-Gated Sodium ChannelsInsecticidesPermethrinPyrethrinsVoltage-Gated Sodium ChannelsAedesHawaiiInsecticide resistanceResistance mutation

Identifiers

PMID41351054
PMCPMC12797669

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