Evidence map›Paper›PMID 41350735›Full record

ArticleJournal of orthopaedic surgery and research2025

Transcriptomic insights into nerve growth factor-associated appositional bone formation in the reparative zone in osteonecrosis of the femoral head.

Yusuke Ayabe, Soichiro Yoshino, Goro Motomura, Ryosuke Yamaguchi, Takeshi Utsunomiya, Kosei Sakamoto, Yasuharu Nakashima

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Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yusuke AyabeDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Soichiro YoshinoDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan. yoshino.soichiro.274@m.kyushu-u.ac.jp.
Goro MotomuraDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Ryosuke YamaguchiDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Takeshi UtsunomiyaDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Kosei SakamotoDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Yasuharu NakashimaDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.

Funding

Japan Society for the Promotion of Science JP23K08699Japan Society for the Promotion of Science JP24K19585Ministry of Health, Labor, and Welfare Research Program on Rare and Intractable Diseases Grant JPMH23FC1045Wellcome Trust FC001045
6 · The paper itself

Abstract

backgroundIn osteonecrosis of the femoral head, appositional bone formation occurs in the reparative zone, where new trabeculae surround necrotic bone, leading to trabecular thickening. However, the underlying molecular mechanisms remain unclear. We previously demonstrated a significant correlation between trabecular thickening and nerve growth factor (NGF) expression in the reparative zone of stage 3 osteonecrosis of the femoral head. This study explored NGF's molecular relationship with appositional bone formation.

methodsRNA sequencing was performed on reparative zone samples from four stage 3 osteonecrosis of the femoral head femoral heads. One to three cylindrical samples (6 mm in diameter) were harvested per head. As controls, three samples were collected from the reparative zone of a single stage 1 osteonecrosis of the femoral head without histological evidence of appositional bone formation. Differentially expressed genes were identified using the DESeq2. Enrichment analysis was performed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases via DAVID. NGF expression was evaluated using immunohistochemistry.

resultsStage 3 samples showed the upregulation of bone formation-related genes, including COL1A1, RUNX2, BMP2, BGLAP, and NGF. KEGG analysis revealed the enrichment of bone-related signaling pathways, such as Wnt, TGF-β, and PI3K-Akt. NGF expression was observed in the reparative zone of stage 3 femoral heads, which exhibited appositional bone formation, but not in stage 1.

conclusionThese findings indicate that the reparative zone exhibiting appositional bone formation shows transcriptional activation of bone formation, accompanied by increased NGF expression. NGF may be involved in the molecular mechanisms underlying appositional bone formation in osteonecrosis of the femoral head, and serve as a potential regulator of this process.

Indexed as

Femur HeadFemur Head NecrosisNerve Growth FactorOsteogenesisTranscriptomeAdultFemaleHumansMaleMiddle AgedNerve Growth FactorNGF protein, humanAppositional bone formationNerve growth factorOsteonecrosis of the femoral headRNA sequencing

Identifiers

PMID41350735
PMCPMC12781920

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