Evidence map›Paper›PMID 41350596›Full record

ArticleCommunications biology2025

Rotational migration in human pancreatic ductal organoids depends on actin and myosin activity.

Gengqiang Xie, Chaity Modak, Olalekan H Usman, Raphael W F Tan, Nicole Coca, Gabriela De Jesus, Yue Julia Wang, D Thirumalai, Xin Li, Jerome Irianto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Gengqiang Xie *Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.ORCID http://orcid.org/0000-0002-2064-4757
Chaity Modak *Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.
Olalekan H UsmanDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.
Raphael W F TanDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.
Nicole CocaDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.
Gabriela De JesusDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.ORCID http://orcid.org/0009-0005-3446-4748
Yue Julia WangDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.ORCID http://orcid.org/0000-0003-2641-6904
D ThirumalaiDepartment of Chemistry, University of Texas at Austin, Austin, TX, USA.ORCID http://orcid.org/0000-0003-1801-5924
Xin LiDepartment of Chemistry, University of Texas at Austin, Austin, TX, USA. xinlee0@gmail.com.ORCID http://orcid.org/0000-0002-2510-2236
Jerome IriantoDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA. jerome.irianto@med.fsu.edu.ORCID http://orcid.org/0000-0002-4804-7225

Funding

Integrated Islet Distribution Program (U24) - 2021U24DK098085 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI Carmella Evans-Molina, Joyce Carol Niland · 2021 to 2026
$17.8M
American Diabetes Association (ADA) 11-22-JDFPM-03Florida Department of Health 21B11Florida Department of Health 23L06National Science Foundation (NSF) PHY 2310639NIDDK NIH HHS U24 DK098085U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) U24DK098085Welch Foundation F-0019
6 · The paper itself

Abstract

Rotational migration is one specific form of collective cell migration when epithelial cells are confined in a spherical geometry, such as in the epithelial acini. This tissue-level rotation motion is crucial for the morphogenesis of multiple epithelial systems. Here, we introduce human pancreatic ductal organoids as a model to study rotational migration. Live imaging revealed the persistent rotation of the organoids over time. By tracking the nuclei, the three-dimensional trajectory of the cellular movement was reconstructed and the velocity of the rotation was quantified. The presence of focal adhesion clusters and prominent actin stress fibers were observed at the basal side of the organoids, suggesting the interactions between the cells and the surrounding extracellular matrix. Finally, our inhibition study showed the dependence of pancreatic ductal organoid rotational migration on myosin activity, actin polymerization, and actin branching. We hope that this model will enable future studies with human primary cells, which are more faithful to normal epithelial cells.

Indexed as

ActinsCell MovementMyosinsOrganoidsPancreatic DuctsEpithelial CellsHumansRotationActinsMyosins

Identifiers

PMID41350596
PMCPMC12680755

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.