Evidence map›Paper›PMID 41350549›Full record

ArticleScientific reports2025

High-frequency ultrasound for microcirculation in metastatic mouse lymph node: contrast-free versus contrast-enhanced imaging.

Kazuki Maeda, Masaaki Omura, Ariunbuyan Sukhbaatar, Tsuyoshi Sugiura, Tetsuya Kodama

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kazuki MaedaLaboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, 4‑1 Seiryo, Aoba, Sendai, Miyagi, 980‑8575, Japan.
Masaaki OmuraFaculty of Engineering, University of Toyama, 3190 Gofuku Toyama, Toyama, 930-8555, Japan.
Ariunbuyan SukhbaatarLaboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, 4‑1 Seiryo, Aoba, Sendai, Miyagi, 980‑8575, Japan.
Tsuyoshi SugiuraBiomedical Engineering Cancer Research Center, Graduate School of Biomedical Engineering, Tohoku University, 4‑1 Seiryo, Aoba, Sendai, Miyagi, 980‑8575, Japan.
Tetsuya KodamaLaboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, 4‑1 Seiryo, Aoba, Sendai, Miyagi, 980‑8575, Japan. kodama@tohoku.ac.jp.

Funding

Japan Society for the Promotion of Science 23H00543Japan Society for the Promotion of Science 25K034895KEIRIN RACE 2025M-329
6 · The paper itself

Abstract

Focal defects are one of the important features for the diagnosis of lymph node (LN) metastasis. In our previous study, an accurate method for detecting contrast agents was proposed. However, conventional B-mode and contrast-enhanced images via ultrasound contrast agents (UCAs) have the limitations of contrast and spatial resolution to visualize the microcirculation, such as focal defects to distinguish between benign and malignant LN. In the present study, we have developed a novel method based on clutter filtering with singular value decomposition (SVD) analysis using time-integrated amplitude envelope (TIAE) in high-frequency 40 MHz ultrasound for high contrast resolution of the microcirculation in LN tissue. A mouse LN was visualized in vivo without and with UCA to compare the contrast enhancement. A metastatic LN model was established with LM8-luc cells of C3H/HeJ-lpr/lpr and MXH54/Mo-lpr/lpr mice. Bioluminescence imaging and pathological observations were also conducted to evaluate tumor growth. It was found that clutter-filtered contrast-enhanced images with UCA could visualize the feature of the microcirculation in the control LN and focal defects in the metastatic LN. Consistent with histological findings of disrupted architecture and cellular heterogeneity, whereas clutter-filtered B-mode images without UCA failed to visualize the vascular circulation. TIAE provided images with high noise resistance, and the calculated vascular area in the LN showed a decreasing trend in the metastatic group compared to the control group. Our framework enables robust visualization and quantification of LN heterogeneity in microcirculation.

Indexed as

Contrast MediaLymphatic MetastasisLymph NodesMicrocirculationAnimalsCell Line, TumorFemaleMiceMice, Inbred C3HUltrasonographyContrast MediaClutter filterContrast-enhanced ultrasoundContrast-freeFocal defectHigh-frequency ultrasoundLymph node metastasisMouse model

Identifiers

PMID41350549
PMCPMC12680702

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.