Evidence map›Paper›PMID 41350468›Full record

ArticleArchives of virology2025

Characterization and genome analysis of the Klebsiella webervirus vB_KpnS_2146-302.

Huasheng Yu, Bohai Du, Hong Peng, Xiao Wei, Xiangna Zhao

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Article in Archives of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Huasheng Yu *Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, China.
Bohai Du *Center for Disease Control and Prevention, Chinese PLA, Beijing, China.
Hong PengCenter for Disease Control and Prevention, Chinese PLA, Beijing, China.
Xiao WeiCenter for Disease Control and Prevention, Chinese PLA, Beijing, China. xwei198672@163.com.
Xiangna ZhaoDepartment of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, China. xnazhao@163.com.ORCID http://orcid.org/0000-0002-1413-7054

Funding

Natural Science Foundation of Beijing Municipality 5202027
6 · The paper itself

Abstract

A novel phage specific for Klebsiella pneumoniae, named vB_KpnS_2146 - 302, was isolated from hospital wastewater. Through transmission electron microscopy observation and genomic analysis, this phage was found to belong to the genus Webervirus in the family Drexlerviridae. Biological studies showed that vB_KpnS_2146 - 302 has a narrow host range and exhibits specific lytic activity against K. pneumoniae. A one-step growth curve showed a latency period of 10 minutes and a burst size of 1125 PFU/cell. The phage remained stable within a pH range of 3-9 and a temperature range of 26-60°C. Whole-genome sequencing analysis showed that the double-stranded DNA genome of vB_KpnS_2146 - 302 is 50,299 bp in length and contains 76 open reading frames. Experiments in which cells were treated with proteinase K or periodate suggested that the carbohydrate structure of K. pneumoniae is involved in the adsorption of this phage. This study shows that phage vB_KpnS_2146 - 302 might be a new candidate for the development of phage therapy against K. pneumoniae infections.

Indexed as

BacteriophagesGenome, ViralKlebsiella pneumoniaeDNA, ViralHost SpecificityOpen Reading FramesPhylogenyWastewaterWhole Genome SequencingDNA, ViralWastewaterGenomic analysisKlebsiella pneumoniaeLytic phageWebervirus

Identifiers

PMID41350468

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.